Characterisation of anthracyclines from a cosmomycin D-producing species of Streptomyces by collisionally-activated dissociation and ion mobility mass spectrometry
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
Cultures of cosmomycin D-producing Streptomyces olindensis ICB20 that were propagated for many generations underwent mutations that resulted in production of a range of related anthracyclines by the bacteria. The anthracyclines that retained the two trisaccharide chains of the parent compound were separated by HPLC. Exact mass determination of these compounds revealed that they differed from cosmomycin D (CosD) in that they contained one to three fewer oxygen atoms (loss of hydroxyl groups). Some of the anthracyclines that were separated by HPLC had the same mass. The location from which the hydroxyl groups had been lost relative to CosD (on the aglycone and/or on the sugar residues) was probed by collisionally-activated dissociation using an electrospray ionisation linear quadrupole ion trap mass spectrometer. The presence of anthracyclines with the same mass, but different structure, was confirmed using an electrospray ionisation travelling wave ion mobility mass spectrometer. University of Wollongong (UoW) University of Wollongong (UoW) University of Sao Paulo (USP) Universidade de São Paulo (USP) Australian Research Council Australian Research Council (ARC) |
Identificador |
EUROPEAN JOURNAL OF MASS SPECTROMETRY, v.15, n.2, p.73-81, 2009 1469-0667 http://producao.usp.br/handle/BDPI/28478 10.1255/ejms.948 |
Idioma(s) |
eng |
Publicador |
IM PUBLICATIONS |
Relação |
European Journal of Mass Spectrometry |
Direitos |
closedAccess Copyright IM PUBLICATIONS |
Palavras-Chave | #anthracycline #linear quadrupole ion trap #MS(n) #travelling wave #ion mobility #etectrospray ionisation mass spectrometry #collisionally-activated dissociation #DNA-BINDING #ANTIBIOTICS #ANALOGS #SUGAR #DRUGS #Physics, Atomic, Molecular & Chemical #Spectroscopy |
Tipo |
article original article publishedVersion |