Disruption of the 2-methylcitric acid cycle and evaluation of poly-3-hydroxybutyrate-co-3-hydroxyvalerate biosynthesis suggest alternate catabolic pathways of propionate in Burkholderia sacchari
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC significantly reduces the bacterial ability to incorporate 3-hydroxyvalerate (3HV) into a biodegradable copolyester accumulated from carbohydrates plus propionate. The efficiency of the mutants in converting propionate to 3HV units (Y(3HV/prp)) increased from 0.09 g.g(-1) to 0.81-0.96 g.g(-1), indicating that acnM and prpC are both essential for growth on propionate. None of the mutations resulted in achievement of the maximum theoretical Y(3HV/prp) (1.35 g.g(-1)). When increasing concentrations of propionate were supplied, decreasing values of Y(3HV/prp) were observed. The results obtained corroborate the hypothesis of the presence of other propionate catabolic pathways in B. sacchari. The 2MCC would be the more operative pathway, but a second pathway, which remains to be elucidated, would assume more importance under propionate concentrations of 1 g.L(-1) or higher. The efficiency in converting propionate to 3HV units can be improved by decreasing the propionate concentrations, owing to the role of the 2MCC. Fundacao de Amparo a Pesquisa do Estado de Sdo Paulo FAPESP[04/14545-0] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sdo Paulo FAPESP[03/00745-4] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) |
Identificador |
CANADIAN JOURNAL OF MICROBIOLOGY, v.55, n.6, p.688-697, 2009 0008-4166 http://producao.usp.br/handle/BDPI/28459 10.1139/W09-018 |
Idioma(s) |
eng |
Publicador |
NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS |
Relação |
Canadian Journal of Microbiology |
Direitos |
restrictedAccess Copyright NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS |
Palavras-Chave | #Burkholderia sacchari #2-methylcitric acid cycle #polyhydroxyalkanoate #3-hydroxyvalerate #propionate catabolism #GRAM-NEGATIVE BACTERIA #ESCHERICHIA-COLI #POLYHYDROXYALKANOIC ACIDS #METHYLCITRATE CYCLE #RALSTONIA-EUTROPHA #DEHYDRATASE PRPD #METABOLISM #GENES #IDENTIFICATION #INVOLVEMENT #Biochemistry & Molecular Biology #Biotechnology & Applied Microbiology #Immunology #Microbiology |
Tipo |
article original article publishedVersion |