Polyhydroxyalkanoates production by actinobacteria isolated from soil


Autoria(s): MATIAS, Fernanda; BONATTO, Diego; PADILLA, Gabriel; RODRIGUES, Maria Filomena de Andrade; HENRIQUES, Joao Antonio Pegas
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

Polyhydroxyalkanoates (PHAs) are biodegradable and renewable polymers produced by a wide range of bacterial groups. New microbial bioprospection approaches have become an important way to find new PHA producers and new synthesized polymers. Over the past years, bacteria belonging to actinomycetes group have become known as PHA producers, such as Nocardia and Rhodococcus species, Kineosphaera limosa Liu et a]. 2002, and, more recently, Streptomyces species. In this paper, we disclose that there are more actinobacteria PHA producers in addition to the genera cited. Some unusual genera, such as Streptoalloteichus, and some genera frequently present in soil, such as Streptacidiphilus, have been found. Thirty-four isolates were able to accumulate poly(3-hydroxybutyrate) and a number of these have traces of poly(3-hydroxyvalerate) when cultivated on glucose or glucose and casein as carbon source. Furthermore, some strains showed traces of medium chain length PHA. Transmission electron microscopy demonstrated that the PHA accumulation occurs in hyphae and spores.

GENOTOX

GENOTOX

Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS)

FAPERGS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP

Identificador

CANADIAN JOURNAL OF MICROBIOLOGY, v.55, n.7, p.790-800, 2009

0008-4166

http://producao.usp.br/handle/BDPI/28454

10.1139/W09-029

http://dx.doi.org/10.1139/W09-029

Idioma(s)

eng

Publicador

NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS

Relação

Canadian Journal of Microbiology

Direitos

restrictedAccess

Copyright NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS

Palavras-Chave #actinobacteria #bioprospection #PHA #PHB #actinomycetes #RECOMBINANT ESCHERICHIA-COLI #POLY-BETA-HYDROXYBUTYRATE #STREPTOMYCES-AUREOFACIENS NRRL-2209 #FLUORESCENT STAIN #STRESS RESISTANCE #NILE RED #ACCUMULATION #ACID #BIOSYNTHESIS #METABOLISM #Biochemistry & Molecular Biology #Biotechnology & Applied Microbiology #Immunology #Microbiology
Tipo

article

original article

publishedVersion