Screening of bacteria to produce polyhydroxyalkanoates from xylose


Autoria(s): LOPES, Mateus Schreiner Garcez; ROCHA, Rafael Costa Santos; ZANOTTO, Sandra Patricia; GOMEZ, Jose Gregorio Cabrera; SILVA, Luiziana Ferreira da
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

Although xylose is a major constituent of lignocellulosic feedstock and the second most abundant sugar in nature, only 22% of 3,152 screened bacterial isolates showed significant growth in xylose in 24 h. Of those 684, only 24% accumulated polyhydroxyalkanoates after 72 h. A mangrove isolate, identified as Bacillus sp. MA3.3, yielded the best results in literature thus far for Gram-positive strains in experiments with glucose and xylose as the sole carbon source. When glucose or xylose were supplied, poly-3-hydroxybutyrate (PHB) contents of cell dry weight were, respectively, 62 and 64%, PHB yield 0.25 and 0.24 g g(-1) and PHB productivity (P(PHB)) 0.10 and 0.06 g l(-1) h(-1). This 40% P(PHB) difference may be related to the theoretical ATP production per 3-hydroxybutyrate (3HB) monomer calculated as 3 mol mol(-1) for xylose, less than half of the ATP/3HB produced from glucose (7 mol mol(-1)). In PHB production using sugar mixtures, all parameters were strongly reduced due to carbon catabolite repression. PHB production using Gram-positive strains is particularly interesting for medical applications because these bacteria do not produce lipopolysaccharide endotoxins which can induce immunogenic reactions. Moreover, the combination of inexpensive substrates and products of more value may lead to the economical sustainability of industrial PHB production.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificador

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v.25, n.10, p.1751-1756, 2009

0959-3993

http://producao.usp.br/handle/BDPI/28444

10.1007/s11274-009-0072-9

http://dx.doi.org/10.1007/s11274-009-0072-9

Idioma(s)

eng

Publicador

SPRINGER

Relação

World Journal of Microbiology & Biotechnology

Direitos

restrictedAccess

Copyright SPRINGER

Palavras-Chave #Xylose #Sugar mixtures #Polyhydroxyalkanoates #Bacillus #Poly-3-hydroxybutyrate #Catabolite repression #GRAM-NEGATIVE BACTERIA #ESCHERICHIA-COLI #PROPIONIC-ACID #BACILLUS #FERMENTATION #COPOLYMERS #STRAINS #ETHANOL #CEPACIA #GENES #Biotechnology & Applied Microbiology
Tipo

article

original article

publishedVersion