iNOS-derived Nitric Oxide Modulates Infection-stimulated Bone Loss
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2008
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Resumo |
Nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) plays an important role in host defense, as well as in inflammation-induced tissue lesions. Here we evaluated the role of NO in bone loss in bacterial infection-induced apical periodontitis by using iNOS-deficient mice (iNOS(-/-)). The iNOS(-/-) mice developed greater inflammatory cell recruitment and osteolytic lesions than WT mice. Moreover, tartrate-resistant acid-phosphatase-positive (TRAP(+)) osteoclasts were significantly more numerous in iNOS-/- mice. Furthermore, the increased bone resorption in iNOS(-/-) mice also correlated with the increased expression of receptor activator NF-kappaB (RANK), stromal-cell-derived factor-1 alpha (SDF-1 alpha/CXCL12), and reduced expression of osteoprotegerin (OPG). These results show that NO deficiency was associated with an imbalance of bone-resorption-modulating factors, leading to severe infection-stimulated bone loss. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) undacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Brazil |
Identificador |
JOURNAL OF DENTAL RESEARCH, v.87, n.12, p.1155-1159, 2008 0022-0345 |
Idioma(s) |
eng |
Publicador |
SAGE PUBLICATIONS INC |
Relação |
Journal of Dental Research |
Direitos |
restrictedAccess Copyright SAGE PUBLICATIONS INC |
Palavras-Chave | #Nitric oxide #osteoclastogenesis #RANK #RANKL #OPG #SDF-1 alpha/CXCL12 #SYNTHASE-DEFICIENT MICE #MARROW STROMAL CELLS #KAPPA-B LIGAND #OSTEOCLAST DIFFERENTIATION #RECEPTOR ACTIVATOR #EXPRESSION #PERIODONTITIS #INVOLVEMENT #INHIBITION #APOPTOSIS #Dentistry, Oral Surgery & Medicine |
Tipo |
article original article publishedVersion |