Critical involvement of Th1-related cytokines in renal injuries induced by ischemia and reperfusion
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
Renal ischemia and reperfusion injury (IRI) is considered an inflammatory syndrome. To move forward in its pathogenesis, we exploited the role of several cytokines on renal damages triggered by IRI. Specifically to evaluate the role of Th1 immune profile in this system, IL-12, IFN-gamma, and IFN-gamma/IL-12 deficient (KO) mice on C57BL/6 background and their controls were subjected to IRI. In each group, blood and kidney samples were harvested. Renal function was evaluated by serum creatinine and renal morphometric analyses. Gene expression of IL-6 and HO-1 were also investigated by Q-PCR. IFN-gamma KO animals presented the highest impairment in renal function compared to controls. Conversely, IL-12 KO animals were absolutely protected and, in a lesser extent, IFN-gamma/IL-12 KO double knockout was also protected from IRI. Gene expression analyses showed higher expression of HO-1, a cytoprotective gene, and IL-6, a pro-inflammatory cytokine, in IFN-gamma deficient animals subjected to IRI. Our results confirm that Th1 related cytokines such as IL-12 and IFN-gamma are critically involved in renal ischemia and reperfusion injury. (C) 2008 Elsevier B.V. All rights reserved. |
Identificador |
INTERNATIONAL IMMUNOPHARMACOLOGY, v.9, n.6, p.668-672, 2009 1567-5769 http://producao.usp.br/handle/BDPI/28299 10.1016/j.intimp.2008.11.012 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE BV |
Relação |
International Immunopharmacology |
Direitos |
restrictedAccess Copyright ELSEVIER SCIENCE BV |
Palavras-Chave | #Acute renal failure #Ischemia and reperfusion injury #Cytokines #IL-12 #Interferon gamma #IL-6 #HO-1 #HEPATIC ISCHEMIA/REPERFUSION INJURY #B-CELL DEFICIENCY #T-CELL #CRESCENTIC GLOMERULONEPHRITIS #HEME OXYGENASE-1 #FAILURE #INFLAMMATION #LYMPHOCYTES #MICE #INTERLEUKIN-12 #Immunology #Pharmacology & Pharmacy |
Tipo |
article proceedings paper publishedVersion |