Local and cardiorenal effects of periodontitis in nitric oxide-deficient hypertensive rats

Objective: In this study we have assessed the renal and cardiac consequences of ligature-induced periodontitis in both normotensive and nitric oxide (NO)-deficient (L-NAME-treated) hypertensive rats. Materials and methods: Oral L-NAME (or water) treatment was started two weeks prior to induction of periodontitis. Rats were sacrificed 3, 7 or 14 days after ligature placement, and alveolar bone loss was evaluated radiographically. Thiobarbituric reactive species (TBARS; a lipid peroxidation index), protein nitrotyrosine (NT; a marker of protein nitration) and myeloperoxidase activity (MPO; a neutrophil marker) were determined in the heart and kidney. Results: In NO-deficient hypertensive rats, periodontitis-induced alveolar bone loss was significantly diminished. In addition, periodontitis-induced cardiac NT elevation was completely prevented by L-NAME treatment. On the other hand L-NAME treatment enhanced MPO production in both heart and kidneys of rats with periodontitis. No changes due to periodontitis were observed in cardiac or renal TBARS content. Conclusions: In addition to mediating alveolar bone loss, NO contributes to systemic effects of periodontitis in the heart and kidney. (C) 2010 Elsevier Ltd. All rights reserved.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

State of Sao Paulo Research Foundation (FAPESP)[2002/00300-0]

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

National Council for Scientific and Technological Development (CNPq)[475549/2004-0]

U.S. National Institutes of Health (NIH)

National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH)[DE16933]

National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH)[DE15566]

U.S. National Institutes of Health (NIH)