Hepatic denervation impairs the assembly and secretion of VLDL-TAG
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
20/10/2012
20/10/2012
2008
|
Resumo |
VLDL secretion is a regulated process that depends on the availability of lipids, apoB and MTP. Our aim was to investigate the effect of liver denervation upon the secretion of VLDL and the expression of proteins involved in this process. Denervation was achieved by applying a 85% phenol solution onto the portal tract, while control animals were treated with 9% NaCl. VLDL secretion was evaluated by the Tyloxapol method. The hepatic concentration of TAG and cholesterol, and the plasma concentration of TAG, cholesterol, VLDL-TAG, VLDL-cholesterol and HDL-cholesterol were measured, as well as mRNA expression of proteins involved in the process of VLDL assembly. Hepatic acinar distribution of MTP and apoB was evaluated by immunohistochemistry. Denervation increased plasma concentration of cholesterol (125.3 +/- 10.1 vs. 67.1 +/- 4.9 mg dL(-1)) and VLDL-cholesterol (61.6 +/- 5.6 vs. 29.4 +/- 3.3 mg dL(-1)), but HDL-cholesterol was unchanged (45.5 +/- 6.1 vs. 36.9 +/- 3.9 mg dL(-1)). Secretion of VLDL-TAG (47.5 +/- 23.8 vs. 148.5 +/- 27.4 mg dL h(-1)) and mRNA expression of CPT I and apoB were reduced (p < 0.01) in the denervated animals. MTP and apoB acinar distribution was not altered in the denervated animals, but the intensity of the reaction was reduced in relation to controls. Copyright (C) 2008 John Wiley & Sons, Ltd. |
Identificador |
CELL BIOCHEMISTRY AND FUNCTION, v.26, n.5, p.557-565, 2008 0263-6484 http://producao.usp.br/handle/BDPI/28120 10.1002/cbf.1476 |
Idioma(s) |
eng |
Publicador |
JOHN WILEY & SONS LTD |
Relação |
Cell Biochemistry and Function |
Direitos |
restrictedAccess Copyright JOHN WILEY & SONS LTD |
Palavras-Chave | #liver #VLDL #denervation #lipoprotein #noradrenaline #LOW-DENSITY-LIPOPROTEIN #TRIGLYCERIDE TRANSFER PROTEIN #B-CONTAINING LIPOPROTEINS #ISOLATED RAT HEPATOCYTES #APOLIPOPROTEIN-C-III #UBIQUITIN-PROTEASOME PATHWAY #DIACYLGLYCEROL ACYLTRANSFERASE #TRANSGENIC MICE #TRIACYLGLYCEROL SECRETION #ENDOPLASMIC-RETICULUM #Biochemistry & Molecular Biology #Cell Biology |
Tipo |
article original article publishedVersion |