Hepatic denervation impairs the assembly and secretion of VLDL-TAG


Autoria(s): TAVARES, Fabio Luis; SEELAENDER, Marilia Cerqueira Leite
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2008

Resumo

VLDL secretion is a regulated process that depends on the availability of lipids, apoB and MTP. Our aim was to investigate the effect of liver denervation upon the secretion of VLDL and the expression of proteins involved in this process. Denervation was achieved by applying a 85% phenol solution onto the portal tract, while control animals were treated with 9% NaCl. VLDL secretion was evaluated by the Tyloxapol method. The hepatic concentration of TAG and cholesterol, and the plasma concentration of TAG, cholesterol, VLDL-TAG, VLDL-cholesterol and HDL-cholesterol were measured, as well as mRNA expression of proteins involved in the process of VLDL assembly. Hepatic acinar distribution of MTP and apoB was evaluated by immunohistochemistry. Denervation increased plasma concentration of cholesterol (125.3 +/- 10.1 vs. 67.1 +/- 4.9 mg dL(-1)) and VLDL-cholesterol (61.6 +/- 5.6 vs. 29.4 +/- 3.3 mg dL(-1)), but HDL-cholesterol was unchanged (45.5 +/- 6.1 vs. 36.9 +/- 3.9 mg dL(-1)). Secretion of VLDL-TAG (47.5 +/- 23.8 vs. 148.5 +/- 27.4 mg dL h(-1)) and mRNA expression of CPT I and apoB were reduced (p < 0.01) in the denervated animals. MTP and apoB acinar distribution was not altered in the denervated animals, but the intensity of the reaction was reduced in relation to controls. Copyright (C) 2008 John Wiley & Sons, Ltd.

Identificador

CELL BIOCHEMISTRY AND FUNCTION, v.26, n.5, p.557-565, 2008

0263-6484

http://producao.usp.br/handle/BDPI/28120

10.1002/cbf.1476

http://dx.doi.org/10.1002/cbf.1476

Idioma(s)

eng

Publicador

JOHN WILEY & SONS LTD

Relação

Cell Biochemistry and Function

Direitos

restrictedAccess

Copyright JOHN WILEY & SONS LTD

Palavras-Chave #liver #VLDL #denervation #lipoprotein #noradrenaline #LOW-DENSITY-LIPOPROTEIN #TRIGLYCERIDE TRANSFER PROTEIN #B-CONTAINING LIPOPROTEINS #ISOLATED RAT HEPATOCYTES #APOLIPOPROTEIN-C-III #UBIQUITIN-PROTEASOME PATHWAY #DIACYLGLYCEROL ACYLTRANSFERASE #TRANSGENIC MICE #TRIACYLGLYCEROL SECRETION #ENDOPLASMIC-RETICULUM #Biochemistry & Molecular Biology #Cell Biology
Tipo

article

original article

publishedVersion