Genotoxic effects of X-rays on keratinized mucosa cells during panoramic dental radiography
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2008
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Resumo |
Objectives: The aim of this study was to evaluate the genotoxic effects of X-rays on epithelial gingival cells during panoramic dental radiography using a differentiated protocol for the micronucleus test. Methods: 40 healthy individuals who underwent this procedure for diagnostic purposes on request from their dentists agreed to participate in this study. All of them answered a questionnaire before the examination. Epithelial gingival cells were obtained from the keratinized mucosa of the upper dental arcade by gentle scraping with a cervical brush immediately before exposure and 10 days later. Cytological preparations were stained according to the Feulgen-Rossenbeck reaction, counterstained with fast green 1% for 1 min and analysed under a light microscope. Micronuclei, nuclear projections (broken eggs) and degenerative nuclear alterations (pyknosis, karyolysis, karyorrhexis and condensed chromatin) were scored. Results: The frequency of micronuclei was significantly higher after exposure (P < 0.05), as were frequencies of nuclear alterations indicate of apoptosis (P < 0.001). Conclusions: These results indicate that X-ray radiation emitted during panoramic dental radiography induces a genotoxic effect on epithelial gingival cells that increases the frequency of chromosomal damage and nuclear alterations indicative of apoptosis. Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB) Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) CNPq, Bahia, Brazil |
Identificador |
DENTOMAXILLOFACIAL RADIOLOGY, v.37, n.7, p.398-403, 2008 0250-832X http://producao.usp.br/handle/BDPI/28116 10.1259/dmfr/56848097 |
Idioma(s) |
eng |
Publicador |
BRITISH INST RADIOLOGY |
Relação |
Dentomaxillofacial Radiology |
Direitos |
restrictedAccess Copyright BRITISH INST RADIOLOGY |
Palavras-Chave | #micro-computed tomography #scanning electron confocal microscopy #internal resorption #quantitative measurement #PERIPHERAL-BLOOD LYMPHOCYTES #HUMAN MICRONUCLEUS PROJECT #BUCCAL EPITHELIAL-CELLS #ORAL-MUCOSA #DNA-DAMAGE #CHROMOSOMAL-ABERRATIONS #RADIATION CARCINOGENESIS #IONIZING-RADIATION #NUCLEAR ANOMALIES #FREQUENCY #Dentistry, Oral Surgery & Medicine #Radiology, Nuclear Medicine & Medical Imaging |
Tipo |
article original article publishedVersion |