Expression Patterns of Cell Adhesion Molecules in Mice`s Lung After Administration of Meso-2,3-Dimercaptosuccinic Acid-Coated Maghemite Nanoparticles


Autoria(s): VALOIS, Caroline R. A.; NUNES, Eloiza S.; JAEGER, Ruy G.; LIMA, Emilia C. D.; MORAIS, Paulo C.; AZEVEDO, Ricardo B.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

We studied the expression pattern of cell adhesion molecules associated to transendothelial migration of leukocytes in different lung`s vascular compartments after administration of a magnetic fluid sample containing maghemite nanoparticles surface-coated with meso-2,3-dimercaptosuccinic acid. The analyses were conducted in mice 4 and 12 h after endovenous administration of the magnetic fluid in control mice. Firstly, the migratory activity of leukocytes after magnetic fluid surface-coated with meso-2,3-dimercaptosuccinic acid administration was confirmed using broncho-alveolar lavage and light microscopy. Then, the expression of cell adhesion molecules in the lung`s vascular compartments was investigated by immunofluorescence microscopy of frozen sections, using antibodies against L-selectin, P-selectin, E-selectin, macrophage antigen-1, and leukocyte function associated antigen-1. L- and P-selectin showed similar pattern of expression in the pulmonary vasculature in animals treated with magnetic fluid and in the control group. In contrast, macrophage antigen-1 and leukocyte function associated antigen-1 were found in capillary only in animals treated with magnetic fluid surface-coated with meso-2,3-dimercaptosuccinic acid administration. In addition, after magnetic fluid administration E-selectin was found in post-capillary sites. Our findings demonstrated that magnetic fluid surface-coated with meso-2,3-dimercaptosuccinic acid administration exhibits modulation effects on expression patterns of E-selectin, macrophage antigen-1, and leukocyte function associated antigen-1 in the lung`s vascular compartments. These findings are very important in a strategy to reduce the potential toxicity of magnetic fluid surface-coated with meso-2,3-dimercaptosuccinic acid administration for medical applications.

MCT/CNPq

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

FINATEC

FINATEC

FAP-DF

Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF)

FINEP

Financiadora de Estudos e Projetos (FINEP)

Identificador

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.9, n.5, p.2846-2855, 2009

1533-4880

http://producao.usp.br/handle/BDPI/28103

10.1166/jnn.2009.049

http://dx.doi.org/10.1166/jnn.2009.049

Idioma(s)

eng

Publicador

AMER SCIENTIFIC PUBLISHERS

Relação

Journal of Nanoscience and Nanotechnology

Direitos

closedAccess

Copyright AMER SCIENTIFIC PUBLISHERS

Palavras-Chave #Integrin #Lung Injury #Magnetic Nanoparticles #Pulmonary Circulation #Selectin #MAGNETIC NANOPARTICLES #NEUTROPHIL EMIGRATION #E-SELECTIN #MIGRATION #LFA-1 #MAC-1 #DMSA #TRANSMIGRATION #DELIVERY #FLUID #Chemistry, Multidisciplinary #Nanoscience & Nanotechnology #Materials Science, Multidisciplinary #Physics, Applied #Physics, Condensed Matter
Tipo

article

original article

publishedVersion