Fasting differentially regulates plasma corticosterone-binding globulin, glucocorticoid receptor, and cell cycle in the gastric mucosa of pups and adult rats


Autoria(s): OGIAS, Daniela; SA, Eunice Ribeiro de Andrade; KASAI, Ariane; MOISAN, Marie-Pierre; ALVARES, Eliana Parisi; GAMA, Patricia
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2010

Resumo

Ogias D, de Andrade Sa ER, Kasai A, Moisan M, Alvares EP, Gama P. Fasting differentially regulates plasma corticosterone-binding globulin, glucocorticoid receptor, and cell cycle in the gastric mucosa of pups and adult rats. Am J Physiol Gastrointest Liver Physiol 298: G117-G125, 2010. First published October 15, 2009; doi:10.1152/ajpgi.00245.2009.-The nutritional status influences gastric growth, and interestingly, whereas cell proliferation is stimulated by fasting in suckling rats, it is inhibited in adult animals. Corticosterone takes part in the mechanisms that govern development, and its effects are regulated in particular by corticosterone-binding globulin (CBG) and glucocorticoid receptor (GR). To investigate whether corticosterone activity responds to fasting and how possible changes might control gastric epithelial cell cycle, we evaluated different parameters during the progression of fasting in 18- and 40-day-old rats. Food restriction induced higher corticosterone plasma concentration at both ages, but only in pups did CBG binding increase after short-and long-term treatments. Fasting also increased gastric GR at transcriptional and protein levels, but the effect was more pronounced in 40-day-old animals. Moreover, in pups, GR was observed in the cytoplasm, whereas, in adults, it accumulated in the nucleus after the onset of fasting. Heat shock protein (HSP) 70 and HSP 90 were differentially regulated and might contribute to the stability of GR and to the high cytoplasmic levels in pups and elevated shuttling in adult rats. As for gastric epithelial cell cycle, whereas cyclin D1 and p21 increased during fasting in pups, in adults, cyclin E slowly decreased, concomitant with higher p27. In summary, we demonstrated that corticosterone function is differentially regulated by fasting in 18-and 40-day-old rats, and such variation might attenuate any possible suppressive effects during postnatal development. We suggest that this mechanism could ultimately increase cell proliferation and allow regular gastric growth during adverse nutritional conditions.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) (Sao Paulo, Brazil)[05/01273-4]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

CNPq[474920/2006-2]

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

CAPES

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Identificador

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, v.298, n.1, p.G117-G125, 2010

0193-1857

http://producao.usp.br/handle/BDPI/28090

10.1152/ajpgi.00245.2009

http://dx.doi.org/10.1152/ajpgi.00245.2009

Idioma(s)

eng

Publicador

AMER PHYSIOLOGICAL SOC

Relação

American Journal of Physiology-gastrointestinal and Liver Physiology

Direitos

restrictedAccess

Copyright AMER PHYSIOLOGICAL SOC

Palavras-Chave #food restriction #p21 #p27 #stomach #SUCKLING RATS #BETA-ISOFORM #EXPRESSION #GROWTH #MICE #PROLIFERATION #MECHANISMS #P27(KIP1) #EPITHELIUM #INTESTINE #Gastroenterology & Hepatology #Physiology
Tipo

article

original article

publishedVersion