Proinsulin C-peptide induces c-Jun N-terminal kinase 1 expression in LEII mouse lung capillary endothelial cells


Autoria(s): FURUYA, Daniela Tomie; ISHII, Tatsuya; KAMIKAWA, Akihiro; SHIMADA, Kohei; MACHADO, Ubiratan Fabres; SAITO, Masayuki; KIMURA, Kazuhiro
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

To characterize the roles of C-peptide in vascular homeostatic processes, we examined the genes regulated by C-peptide in LEII mouse lung microvascular endothelial cells. Treatment of the cells with C-peptide increased the expression of c-Jun N-terminal kinase 1 (JNK1) mRNA dose-dependently, accompanied by an increase in JNK1 protein content. Prior treatment of the cells with PD98059, an ERK kinase inhibitor or SB203580, a p38MAPK inhibitor, abrogated the C-peptide-elicited JNK1 mRNA expression. These results indicate that C-peptide increases JNK1 protein levels, possibly through ERK- and p38MAPK-dependent activation of JNK. gene transcription.

Japan Society for the Promotion of Science (JSPS)

Japan Society for the Promotion of Science (JSPS)

Mombusho Scholarship for International Students

Mombusho Scholarship for International Students

Identificador

JAPANESE JOURNAL OF VETERINARY RESEARCH, v.57, n.3, p.163-167, 2009

0047-1917

http://producao.usp.br/handle/BDPI/28059

http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000272640800003&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord

Idioma(s)

eng

Publicador

HOKKAIDO UNIV

Relação

Japanese Journal of Veterinary Research

Direitos

restrictedAccess

Copyright HOKKAIDO UNIV

Palavras-Chave #C-peptide #diabetes mellitus #JNK #SWISS 3T3 FIBROBLASTS #RENAL TUBULAR CELLS #MAP KINASES #PROTEIN #TRANSCRIPTION #GENE #MICROCIRCULATION #DYSFUNCTION #ACTIVATION #PATHWAY #Veterinary Sciences
Tipo

article

original article

publishedVersion