Moderate exercise changes synaptic and cytoskeletal proteins in motor regions of the rat brain


Autoria(s): FERREIRA, Ana F. B.; REAL, Caroline C.; RODRIGUES, Alice C.; ALVES, Adilson S.; BRITTO, Luiz R. G.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2010

Resumo

Physical exercise is known to enhance brain function in several aspects. We evaluated the acute effects of a moderate forced exercise protocol on synaptic proteins, namely synapsin 1 (SYN) and synaptophysin (SYP), and structural proteins (neurofilaments, NFs) in rat brain regions related to motor function and often affected by neurodegenerative disorders. Immunohistochemistry, Western blotting and real-time PCR were used to analyze the expression of those proteins after 3, 7 and 15 days of exercise (EX3, EX7 and EX15). In the cerebellum, increase of SYN was observed at EX7 and EX15 and of NF68 at EX3. In the substantia nigra, increases of protein levels were observed for NF68 and NF160 at EX3. In the striatum, there was an increase of SYN at EX3 and EX7, of SYP at EX7 and of NF68 at EX3. In the cortex, decreased levels of NF68 and NF160 were observed at EX3, followed by an increase of NF68 at EX15. In the reticular formation, all NF proteins were increased at EX15. The mRNA data for each time-point and region also revealed significant exercise-related changes of SYN, SYP and NF expression. These results suggest that moderate physical exercise modulates synaptic and structural proteins in motor brain areas, which may play an important role in the exercise-dependent brain plasticity. (C) 2010 Elsevier B.V. All rights reserved.

FAPESP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

CNPq (Brazil)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identificador

BRAIN RESEARCH, v.1361, p.31-42, 2010

0006-8993

http://producao.usp.br/handle/BDPI/28007

10.1016/j.brainres.2010.09.045

http://dx.doi.org/10.1016/j.brainres.2010.09.045

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Brain Research

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Physical exercise #Plasticity #Synapsin I #Synaptophysin #Neurofilaments #ADULT HIPPOCAMPAL NEUROGENESIS #PARVALBUMIN-POSITIVE NEURONS #LONG-TERM DEPRESSION #SPINAL-CORD-INJURY #VOLUNTARY EXERCISE #PHYSICAL-ACTIVITY #DENTATE GYRUS #ENVIRONMENTAL ENRICHMENT #CELL-PROLIFERATION #GENE-EXPRESSION #Neurosciences
Tipo

article

original article

publishedVersion