Effect of Fish Oil Supplementation for Two Generations on Changes of Lymphocyte Function Induced by Walker 256 Cancer Cachexia in Rats


Autoria(s): FOLADOR, Alessandra; LIMA-SALGADO, Thais Martins de; HIRABARA, Sandro Massao; AIKAWA, Julia; YAMAZAKI, Ricardo K.; MARTINS, Edgair F.; OLIVEIRA, Heloisa Helena P. de; PIZATTO, Nathalia; KANUNFRE, Carla C.; PERES, Carmem M.; FERNANDES, Luiz C.; Curi, Rui
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

Fish oil supplementation has been shown to improve the cachectic state of tumor-bearing animals and humans. Our previous study showed that fish oil supplementation (1 g per kg body weight per day) for 2 generations had anticancer and anticachetic effects in Walker 256 tumor-bearing rats as demonstrated by reduced tumor growth and body weight loss and increased food intake and survival. In this study, the effect of fish oil supplementation for 2 generations on membrane integrity, proliferation capacity, and CD4/CD8 ratio of lymphocytes isolated from mesenteric lymph nodes, spleen, and thymus of Walker 256 tumor-bearing animals was investigated. We also determined fish oil effect on plasma concentration and ex vivo production of cytokines [tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-4 (IL-4), IL-6, and IL-10]. Lymphocytes from thymus of tumor-bearing rats presented lower viability, but this change was abolished by fish oil supplementation. Tumor growth increased proliferation of lymphocytes from all lymphoid organs, and fish oil supplementation abolished this effect. Ex vivo production of TNF-alpha and IL-6 was reduced in supplemented animals, but IL-4 and IL-10 secretion was stimulated in both nontumor and tumor-bearing rats. IL-10 and IFN-gamma plasma levels was also decreased in supplemented animals. These results suggest that the anticachetic effects of fish oil supplementation for a long period of time (2 generations) in Walker 256 tumor-bearing rats may be associated to a decrease in lymphocyte function as demonstrated by reduced viability, proliferation capacity, and cytokine production.

FAPESP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

CNPq

CAPES

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Identificador

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, v.61, n.5, p.670-679, 2009

0163-5581

http://producao.usp.br/handle/BDPI/27917

10.1080/01635580902825548

http://dx.doi.org/10.1080/01635580902825548

Idioma(s)

eng

Publicador

LAWRENCE ERLBAUM ASSOC INC-TAYLOR & FRANCIS

Relação

Nutrition and Cancer-an International Journal

Direitos

restrictedAccess

Copyright LAWRENCE ERLBAUM ASSOC INC-TAYLOR & FRANCIS

Palavras-Chave #POLYUNSATURATED FATTY-ACIDS #TUMOR-NECROSIS-FACTOR #DIETARY SUPPLEMENTATION #EICOSAPENTAENOIC ACID #PANCREATIC-CANCER #WEIGHT-LOSS #N-3 #IMMUNITY #GROWTH #MICE #Oncology #Nutrition & Dietetics
Tipo

article

original article

publishedVersion