Microcystins -LA, -YR, and -LR action on neutrophil migration
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
Microcystins (MCs) produced by some freshwater cyanobacterial species possess potent liver toxicity as evidenced by acute neutrophil infiltration. Here, we investigate the ability of three structurally distinct toxins (MC-LA, MC-LR, and MC-YR) to evoke neutrophil recruitment per se and their effects on migration pathways. Intravital Microscopic Studies showed that topical application of only MC-LR enhanced the numbers of rolling and adhered leukocytes in the endothelium of postcapillary mesenteric venules. The latter effects may be dependent upon induction of the synthesis and expression Of L-selectin and beta(2)-integrin in neutrophils, as assessed by flow cytometry and RT-PCR, respectively. Conversely, the three toxins promoted direct locomotion of neutrophils and enhanced their migration in response to NO, as measured by Boyden chamber assays, and increased intracellular calcium, a messenger in the chemotaxic process. In conclusion, our results show that MCs act on specific pathways of neutrophil recruitment, indicating their potential effect on neutrophils activation. (C) 2009 Elsevier Inc. All rights reserved. Brazilian research funding agency Conselho Nacional cle Desenvolvimento e Tecnologia (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Coordena o de Aperfei oamento cle Pessoal cle Nivel Superior (CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) |
Identificador |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.382, n.1, p.9-14, 2009 0006-291X http://producao.usp.br/handle/BDPI/27909 10.1016/j.bbrc.2009.02.009 |
Idioma(s) |
eng |
Publicador |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
Relação |
Biochemical and Biophysical Research Communications |
Direitos |
restrictedAccess Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE |
Palavras-Chave | #Microcystin #Neutrophils #Migration #Rolling #Adherence #Adhesion molecules #Calcium influx #MITOCHONDRIAL PERMEABILITY TRANSITION #OXIDATIVE STRESS #RAT HEPATOCYTES #LIVER-INJURY #KAPPA-B #BRAZIL #CYANOBACTERIA #INTOXICATION #ACTIVATION #EXPOSURE #Biochemistry & Molecular Biology #Biophysics |
Tipo |
article original article publishedVersion |