The reactions of specific neuron types to intestinal ischemia in the guinea pig enteric nervous system
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
Damage following ischemia and reperfusion (I/R) is common in the intestine and can be caused during abdominal surgery, in several disease states and following intestinal transplantation. Most studies have concentrated on damage to the mucosa, although published evidence also points to effects on neurons. Moreover, alterations of neuronally controlled functions of the intestine persist after I/R. The present study was designed to investigate the time course of damage to neurons and the selectivity of the effect of I/R damage for specific types of enteric neurons. A branch of the superior mesenteric artery supplying the distal ileum of anesthetised guinea pigs was occluded for 1 h and the animals were allowed to recover for 2 h to 4 weeks before tissue was taken for the immunohistochemical localization of markers of specific neuron types in tissues from sham and I/R animals. The dendrites of neurons with nitric oxide synthase (NOS) immunoreactivity, which are inhibitory motor neurons and interneurons, were distorted and swollen by 24 h after I/R and remained enlarged up to 28 days. The total neuron profile areas (cell body plus dendrites) increased by 25%, but the sizes of cell bodies did not change significantly. Neurons of type II morphology (intrinsic primary afferent neurons), revealed by NeuN immunoreactivity, were transiently reduced in cell size, at 24 h and 7 days. These neurons also showed signs of minor cell surface blebbing. Calretinin neurons, many of which are excitatory motor neurons, were unaffected. Thus, this study revealed a selective damage to NOS neurons that was observed at 24 h and persisted up to 4 weeks, without a significant change in the relative numbers of NOS neurons. |
Identificador |
ACTA NEUROPATHOLOGICA, v.118, n.2, p.261-270, 2009 0001-6322 http://producao.usp.br/handle/BDPI/27863 10.1007/s00401-009-0549-5 |
Idioma(s) |
eng |
Publicador |
SPRINGER |
Relação |
Acta Neuropathologica |
Direitos |
restrictedAccess Copyright SPRINGER |
Palavras-Chave | #Enteric nervous system #Ischemia/reperfusion #Nitric oxide synthase #Small intestine #RAT SMALL-INTESTINE #REPERFUSION INJURY #CELL-DEATH #MYENTERIC PLEXUS #INTERSTITIAL-CELLS #EPITHELIAL-CELLS #NITRIC-OXIDE #IN-VIVO #ISCHEMIA/REPERFUSION #MOTILITY #Clinical Neurology #Neurosciences #Pathology |
Tipo |
article original article publishedVersion |