Reactive oxygen and nitrogen species balance in the determination of thyroid hormones-induced cardiac hypertrophy mediated by renin-angiotensin system
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2011
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Resumo |
Role of reactive oxygen species (ROS)/nitric oxide (NO) balance and renin-angiotensin system in mediating cardiac hypertrophy in hyperthyroidism was evaluated in an in vivo and in vitro experimental model. Male Wistar rats were divided into four groups: control, thyroid hormone, vitamin E (or Trolox, its hydrosoluble analogue), thyroid hormone + vitamin E. Angiotensin II receptor (AT1/AT2) gene expression, immunocontent of AT1/AT2 receptors, angiotensinogen, NADPH oxidase (Nox2), and nitric oxide synthase isoforms, as well as ROS concentration (hydrogen peroxide and superoxide anion) were quantified in myocardium. Thyroid hormone increased ROS and NO metabolites, iNOS, nNOS and eNOS isoforms and it was accompanied by cardiac hypertrophy. AT1/AT2 expression and the immunocontent of angiotensinogen and Nox2 were enhanced by thyroid hormone. Antioxidants reduced ROS levels, Nox2, AT1/AT2, NOS isoforms and cardiac hypertrophy. In conclusion, ROS/NO balance may play a role in the control of thyroid hormone-induced cardiac hypertrophy mediated by renin-angiotensin system. (C) 2011 Elsevier Ireland Ltd. All rights reserved. CNPq, Brazilian Research Agency Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) CAPES, Brazilian Research Agency Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) SCT-RS, Brazilian Research Agency SCT-RS, Brazilian Research Agency FAPERGS, Brazilian Research Agency Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) Northern Ontario School of Medicine Northern Ontario School of Medicine |
Identificador |
MOLECULAR AND CELLULAR ENDOCRINOLOGY, v.333, n.1, p.78-84, 2011 0303-7207 http://producao.usp.br/handle/BDPI/27829 10.1016/j.mce.2010.12.018 |
Idioma(s) |
eng |
Publicador |
ELSEVIER IRELAND LTD |
Relação |
Molecular and Cellular Endocrinology |
Direitos |
restrictedAccess Copyright ELSEVIER IRELAND LTD |
Palavras-Chave | #Renin-angiotensin system #Nitric oxide #Superoxide anion #Thyroid hormones #Cardiac hypertrophy #NITRIC-OXIDE #HYDROGEN-PEROXIDE #EXPERIMENTAL HYPERTHYROIDISM #CARDIOVASCULAR-SYSTEM #OXIDATIVE STRESS #GENE-EXPRESSION #RAT #THYROXINE #DISEASE #HEART #Cell Biology #Endocrinology & Metabolism |
Tipo |
article original article publishedVersion |