Basic aminopeptidase activity is an emerging biomarker in collagen-induced rheumatoid arthritis
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2011
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Resumo |
The objective of this study was to investigate the catalytic activity of basic aminopeptidase (APB) and its association with periarticular edema and circulating tumor necrosis factor (TNF)-alpha and type II collagen (CII) antibodies (AACII) in a rat model of rheumatoid arthritis (RA) induced by CII (CIA). Edema does not occur in part of CH-treated, even when AACII is higher than in control. TNF-alpha is detectable only in edematous CII-treated. APB in synovial membrane is predominantly a membrane-bound activity also present in soluble form and with higher activity in edematous than in non-edematous CH-treated or control. Synovial fluid and blood plasma have lower APB in non-edematous than in edematous CII-treated or control. In peripheral blood mononuclear cells (PBMCs) the highest levels of APB are found in soluble form in control and in membrane-bound form in non-edematous CII-treated. CII treatment distinguishes two categories of rats: one with arthritic edema, high AACII, detectable TNF-alpha, high soluble and membrane-bound APB in synovial membrane and low APB in the soluble fraction of PBMCs, and another without edema and with high AACII, undetectable TNF-alpha, low APB in the synovial fluid and blood plasma and high APB in the membrane-bound fraction of PBMCs. Data suggest that APB and CIA are strongly related. (C) 2011 Elsevier B.V. All rights reserved. FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil)[09/17613-0] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil)[301417/2008-3] Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil) |
Identificador |
REGULATORY PEPTIDES, v.167, n.2/Mar, p.215-221, 2011 0167-0115 http://producao.usp.br/handle/BDPI/27715 10.1016/j.regpep.2011.02.012 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE BV |
Relação |
Regulatory Peptides |
Direitos |
restrictedAccess Copyright ELSEVIER SCIENCE BV |
Palavras-Chave | #Aminopeptidase #Rat model #Inflammatory arthritis #Rheumatoid arthritis #Biomarker #LEUKOTRIENE A(4) HYDROLASE #RENIN-ANGIOTENSIN SYSTEM #SYNOVIAL-FLUID #PROTEIN #CELLS #OSTEOARTHRITIS #PATHOGENESIS #MACROPHAGES #ACTIVATION #MECHANISMS #Endocrinology & Metabolism #Physiology |
Tipo |
article original article publishedVersion |