Striatum brain-derived neurotrophic factor levels are decreased in dystrophin-deficient mice


Autoria(s): COMIM, Clarissa M.; TUON, Lisiane; STERTZ, Laura; VAINZOF, Mariz; KAPCZINSKI, Flavio; QUEVEDO, Joao
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

Brain dystrophin is enriched in the postsynaptic densities of pyramidal neurons specialized regions of the subsynaptic cytoskeletal network, which are critical for synaptic transmission and plasticity. Lack of dystrophin in brain structures have been involved with impaired cognitive functions. The brain-derived neurotrophic factor (BDNF) is a regulator of neuronal survival, fast synaptic transmission, and activity-dependent synaptic plasticity. The present study investigated BDNF protein levels by Elisa analysis in prefrontal cortex, cerebellum, hippocampus, striatum and cortex tissues from male dystrophic mdx (n = 5) and normal C57BL10 mouse (n = 5). We observed that the mdx mouse display diminution in BDNF levels in striatum (t = 6.073; df = 6; p = 0.001), while a tendency of decrease in BDNF levels was observed in the prefrontal cortex region (t = 1.962; df = 6; p = 0.096). The cerebellum (t = 1.258; df = 7; p = 0.249), hippocampus (t = 0.631; df = 7; p = 0.548) and cortex (t = 0.572; df = 7; p = 0.586) showed no significant alterations as compared to wt mouse. In conclusion, we demonstrate that only striatum decreased BDNF levels compared with wild-type (wt) mouse, differently to the other areas of the brain. This dystrophin deficiency may be affecting BDNF levels in striatum and contributing, in part, in memory storage and restoring. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

CNPq

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Instituto Cerebro e Mente

Instituto Cerebro e Mente

UNESC

United Nations Educational, Scientific and Cultural Organization (UNESCO)

Identificador

NEUROSCIENCE LETTERS, v.459, n.2, p.66-68, 2009

0304-3940

http://producao.usp.br/handle/BDPI/27540

10.1016/j.neulet.2009.04.065

http://dx.doi.org/10.1016/j.neulet.2009.04.065

Idioma(s)

eng

Publicador

ELSEVIER IRELAND LTD

Relação

Neuroscience Letters

Direitos

restrictedAccess

Copyright ELSEVIER IRELAND LTD

Palavras-Chave #BDNF #mdx mice #Central nervous system #DUCHENNE MUSCULAR-DYSTROPHY #CENTRAL-NERVOUS-SYSTEM #MDX MICE #NEURONS #SCHIZOPHRENIA #LOCALIZATION #UTROPHIN #CORTEX #Neurosciences
Tipo

article

original article

publishedVersion