Biological effects of insulin on murine melanoma cells and fish erythrophoroma cells: A comparative study
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2008
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Resumo |
Insulin is the hormone that plays an essential role in metabolism and mitosis of normal and tumor cells, exerting its pleiotropic effects through binding to specific membrane receptors and promoting the phosphorylation of tyrosine residues of the receptor itself and of other components of the signaling pathway. The aim of this study was to investigate the effects of insulin on melanogenesis and cell growth in three different cell lines: the goldfish GEM-81 erythrophoroma cells (undifferentiated and differentiated with 1.5% dimethylsulfoxide-DMSO), and the murine B16F10 and Cloudman S91 melanoma cells. Undifferentiated GEM-81 and B16F10 cells responded to insulin with a small increase of cell proliferation, whereas S91 cells responded with a decrease of growth. In the two mammalian cell lines, and in DMSO-differentiated GEM-81 cells, the hormone strongly inhibited melanogenesis, by decreasing tyrosinase activity. In undifferentiated GEM-81 cells, insulin had no effect on tyrosinase activity. An increase in the tyrosine phosphorylation status of pp 185 (insulin receptor substrate 1 and 2-IRS-1/2) phosphorylation degree was observed in S91 mouse melanoma and in differentiated GEM-81 erythrophoroma cells, suggesting that this specific protein was maintained during transformation process and participates in insulin signaling. Our results imply an ancient and diverse history of the insulin signaling system in vertebrate pigment cells. (C) 2008 Elsevier Inc. All rights reserved. |
Identificador |
GENERAL AND COMPARATIVE ENDOCRINOLOGY, v.156, n.2, p.218-223, 2008 0016-6480 http://producao.usp.br/handle/BDPI/27460 10.1016/j.ygcen.2008.02.001 |
Idioma(s) |
eng |
Publicador |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
Relação |
General and Comparative Endocrinology |
Direitos |
restrictedAccess Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE |
Palavras-Chave | #pigment cells #insulin #melanogenesis #cell proliferation #signaling pathway #NORMAL HUMAN MELANOCYTES #GOLDFISH ERYTHROPHOROMA #RESISTANT VARIANTS #SIGNALING SYSTEM #MELANIZED CELLS #RECEPTOR #GROWTH #PEPTIDES #PROLIFERATION #LINE #Endocrinology & Metabolism |
Tipo |
article original article publishedVersion |