Exposure to ambient levels of particles emitted by traffic worsens emphysema in mice
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
19/10/2012
19/10/2012
2009
|
Resumo |
Objectives: We investigated effects of chronic exposure (2 months) to ambient levels of particulate matter (PM) on development of protease-induced emphysema and pulmonary remodeling in mice. Methods: Balb/c mice received nasal drop of either papain or normal saline and were kept in two exposure chambers situated in an area with high traffic density. One of them received ambient air and the other had filters for PM. Results: mean concentration of PM10 was 2.68 +/- 0.38 and 33.86 +/- 2.09 mu g/m(3), respectively, in the filtered and ambient air chambers (p<0.001). After 2 months of exposure, lungs from papain-treated mice kept in the chamber with ambient air presented greater values of mean linear intercept, an increase in density of collagen fibers in alveolar septa and in expression of 8-isoprostane (p = 0.002, p < 0.05 and p = 0.002, respectively, compared to papain-treated mice kept in the chamber with filtered air). We did not observe significant differences between these two groups in density of macrophages and in amount of cells expressing matrix metalloproteinase-12. There were no significant differences in saline-treated mice kept in the two chambers. Conclusions: We conclude that exposure to urban levels of PM worsens protease-induced emphysema and increases pulmonary remodeling. We suggest that an increase in oxidative stress induced by PM exposure influences this response. These pulmonary effects of PM were observed only in mice with emphysema. (C) 2009 Elsevier Inc. All rights reserved. |
Identificador |
ENVIRONMENTAL RESEARCH, v.109, n.5, p.544-551, 2009 0013-9351 http://producao.usp.br/handle/BDPI/26914 10.1016/j.envres.2009.03.002 |
Idioma(s) |
eng |
Publicador |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
Relação |
Environmental Research |
Direitos |
restrictedAccess Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE |
Palavras-Chave | #Air pollutants #Elastases #Oxidants #Remodeling #OBSTRUCTIVE PULMONARY-DISEASE #ELASTASE-INDUCED EMPHYSEMA #PARTICULATE AIR-POLLUTION #OXIDATIVE STRESS #SAO-PAULO #IN-VIVO #ULTRAFINE PARTICLES #OXIDANT STRESS #ANIMAL-MODELS #FATAL ASTHMA #Environmental Sciences #Public, Environmental & Occupational Health |
Tipo |
article proceedings paper publishedVersion |