Conceptus-mediated endometrial vascular changes during early pregnancy in mares: an anatomic, histomorphometric, and vascular endothelial growth factor receptor system immunolocalization and gene expression study


Autoria(s): SILVA, L. A.; KLEIN, C.; EALY, A. D.; SHARP, D. C.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2011

Resumo

This work examined how the conceptus modulates endometrial tissue remodeling and vascular development prior to implantation in mares. A macroscopic uterine examination was completed at day 21 of pregnancy. In situ morphology revealed that the endometrium involved in encroachment is restricted to the dorsal endometrium immediately overlying the yolk sac. The amount of stromal area occupied by blood vessels and the number of endometrial glands were increased during early pregnancy. Endometrial histomorphometry as well as the endometrial mRNA abundance and immunolocalization of VEGF, VEGFR1, VEGFR2, and Ki-67 was completed at days 14 and 21 of pregnancy, at day 10 of the estrous cycle, and during estrus. No obvious differences in VEGF and VEGFR1 protein localization were detected between pregnant and cycling mares but differential staining pattern for VEGFR2 and Ki-67 was observed. VEGFR2 localized to luminal and glandular epithelium of pregnant mares, while luminal epithelium was negative in cycling mares. Ki-67 staining was weak during the luteal phase but exhibited prominent luminal epithelium staining during estrus. In pregnant mares, all endometrial layers were Ki-67 positive. Quantitative RT-PCR revealed a greater abundance of VEGF mRNA during pregnancy. VEGFR2 transcript abundance was greatest in pregnant mares on day 21. This study supports the concept that the conceptus plays an active role in directing vasculogenesis within the uterus and thereby establishing hemotrophic nutrition that supports pregnancy after implantation. Reproduction (2011) 142 593-603

CAPES (Brazil)

[P1-LS-07]

[P2-LS-07]

Identificador

REPRODUCTION, v.142, n.4, p.593-603, 2011

1470-1626

http://producao.usp.br/handle/BDPI/26776

10.1530/REP-11-0149

http://dx.doi.org/10.1530/REP-11-0149

Idioma(s)

eng

Publicador

BIOSCIENTIFICA LTD

Relação

Reproduction

Direitos

restrictedAccess

Copyright BIOSCIENTIFICA LTD

Palavras-Chave #EARLY EQUINE CONCEPTUS #ESTROUS-CYCLE #BLOOD-FLOW #FACTOR VEGF #UTERINE #ANGIOGENESIS #UTERUS #PERMEABILITY #IMPLANTATION #ORIENTATION #Developmental Biology #Reproductive Biology
Tipo

article

original article

publishedVersion