TNF-alpha Promotes an Odontoblastic Phenotype in Dental Pulp Cells
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2009
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Resumo |
Dental pulp cells can differentiate toward an odontoblastic phenotype to produce reparative dentin beneath caries lesions. However, the mechanisms involved in pulp cell differentiation under pro-inflammatory stimuli have not been well-explored. Thus, we hypothesized that the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) could be a mediator involved in dental pulp cell differentiation toward an odontoblastic phenotype. We observed that TNF-alpha-challenged pulp cells exhibited increased mineralization and early and increased expression of dentin phosphoprotein (DPP), dentin sialoprotein (DSP), dentin matrix protein-1, and osteocalcin during a phase of reduced matrix metalloproteinase (MMP) expression. We investigated whether these events were related and found that p38, a mitogen-activated protein kinase, differentially regulated MMP-1 and DSP/DPP expression and mediated mineralization upon TNF-alpha treatment. These findings indicate that TNF-alpha stimulates differentiation of dental pulp cells toward an odontoblastic phenotype via p38, while negatively regulating MMP-1 expression. CAPES Foundation Fellowship[0668/07-9] [NIH-R01-13725] |
Identificador |
JOURNAL OF DENTAL RESEARCH, v.88, n.4, p.339-344, 2009 0022-0345 http://producao.usp.br/handle/BDPI/26435 10.1177/0022034509334070 |
Idioma(s) |
eng |
Publicador |
SAGE PUBLICATIONS INC |
Relação |
Journal of Dental Research |
Direitos |
restrictedAccess Copyright SAGE PUBLICATIONS INC |
Palavras-Chave | #dental pulp cells #tumor necrosis factor-alpha #dentin sialophosphoprotein #matrix metalloproteinase-1 #p38 MAPK #NECROSIS-FACTOR-ALPHA #PERIODONTAL-LIGAMENT CELLS #STEM-CELLS #ALKALINE-PHOSPHATASE #GENE-EXPRESSION #MINERALIZATION MARKERS #DENTINOGENESIS #PROTEINS #KINASE #DIFFERENTIATION #Dentistry, Oral Surgery & Medicine |
Tipo |
article original article publishedVersion |