Stromal-derived factor-1 alpha (CXCL12) levels increase in periodontal disease


Autoria(s): HAVENS, Aaron M.; CHIU, Evonne; TABA JR., Mario; WANG, Jincheng; SHIOZAWA, Yusuke; JUNG, Younghun; TAICHMAN, L. Susan; D`SILVA, Nisha J.; GOPALAKRISHNAN, R.; WANG, CunYu; GIANNOBILE, William V.; TAICHMAN, Russell S.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Background: The CXC chemokine receptor 4 (CXCR4) and its ligand, stromal cell-derived factor-1 (SDF-1 alpha or CXC chemokine ligand 12) are involved in the trafficking of leukocytes into and out of extravascular tissues. The purpose of this study was to determine whether SDF-1 alpha secreted by host cells plays a role in recruiting inflammatory cells into the periodontia during local inflammation. Methods: SDF-1 alpha levels were determined by enzyme-linked immunosorbent assay in gingival crevicular fluid (GCF) of 24 individuals with periodontitis versus healthy individuals in tissue biopsies and in a preclinical rat model of Porphyromonas gingivalis lipopolysaccharide-induced experimental bone loss. Neutrophil chemotaxis assays were also used to evaluate whether SDF-1 alpha plays a role in the recruitment of host cells at periodontal lesions. Results: Subjects with periodontal disease had higher levels of SDF-1 alpha in their GCF compared to healthy subjects. Subjects with periodontal disease who underwent mechanical therapy demonstrated decreased levels of SDF-1 alpha. Immunohistologic staining showed that SDF-1 alpha and CXCR4 levels were elevated in samples obtained from periodontally compromised individuals. Similar results were observed in the rodent model. Neutrophil migration was enhanced in the presence of SDF-1 alpha, mimicking immune cell migration in periodontal lesions. Conclusions: SDF-1 alpha may be involved in the immune defense pathway activated during periodontal disease. Upon the development of diseased tissues, SDF-1 alpha levels increase and may recruit host defensive cells into sites of inflammation. These studies suggest that SDF-1 alpha may be a useful biomarker for the identification of periodontal disease progression.

Identificador

JOURNAL OF PERIODONTOLOGY, v.79, n.5, p.845-853, 2008

0022-3492

http://producao.usp.br/handle/BDPI/26409

10.1902/jop.2008.070514

http://dx.doi.org/10.1902/jop.2008.070514

Idioma(s)

eng

Publicador

AMER ACAD PERIODONTOLOGY

Relação

Journal of Periodontology

Direitos

restrictedAccess

Copyright AMER ACAD PERIODONTOLOGY

Palavras-Chave #chemokine #CXCL12 #CXCR4 #inflammation #periodontitis #SDF-1 alpha #HEMATOPOIETIC PROGENITOR CELLS #FACTOR-I #BONE-MARROW #STIMULATING FACTOR #ENDOTHELIAL-CELLS #ALVEOLAR BONE #EXPRESSION #CXCR4 #CHEMOKINES #SECRETION #Dentistry, Oral Surgery & Medicine
Tipo

article

original article

publishedVersion