In vitro cytotoxicity of dental alloys and cpTi obtained by casting
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2008
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Resumo |
Background: This study aimed to compare the cytotoxicity of base-metal dental alloys and to evaluate if the casting method could influence their cytotoxicity. Methods: Disks of base-metal dental alloys were cast by two methods: plasma, under argon atmosphere, injected by vacuum-pressure; and oxygen-gas flame, injected by centrifugation, except Ti-6Al-4V and commercially pure titanium (cpTi), cast only by plasma. SCC9 cells were cultured in culture media D-MEM/Ham`s F12 supplemented, at 37 degrees C in a humidified atmosphere of 5% carbon dioxide and 95% air, on the previously prepared disks. At subconfluence in wells without disks (control), cell number and viability were evaluated. Results: In plasma method, cpTi and Ti-6Al-4V were similar to control and presented higher number of cells than all other alloys, followed by Ni-Cr. In oxygen-gas name method, all alloys presented fewer cells than control. Ni-Cr presented more cells than any other alloy, followed by Co-Cr-Mo-W which presented more cells than Ni-Cr-Ti, Co-Cr-Mo, and Ni-Cr-Be. There were no significant differences between casting methods related to cell number. Cell viability was not affected by either chemical composition or casting methods. Conclusion: cpTi and Ti-6Al-4V were not cytotoxic while Ni-Cr-Be was the most cytotoxic among tested alloys. The casting method did not affect cytotoxicity of the alloys. (c) 2007 Wiley Periodicals, Inc. |
Identificador |
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v.85B, n.2, p.504-508, 2008 1552-4973 http://producao.usp.br/handle/BDPI/26384 10.1002/jbm.b.30972 |
Idioma(s) |
eng |
Publicador |
WILEY-LISS |
Relação |
Journal of Biomedical Materials Research Part B-applied Biomaterials |
Direitos |
restrictedAccess Copyright WILEY-LISS |
Palavras-Chave | #biocompatibility/soft tissue #titanium (alloys) #cell culture #cytotoxicity #dental/craniofacial material #TITANIUM-ALLOYS #CORROSION BEHAVIOR #BIOCOMPATIBILITY #RESTORATIONS #METALS #TESTS #Engineering, Biomedical #Materials Science, Biomaterials |
Tipo |
article original article publishedVersion |