An upgrade on the rabbit model of anthracycline-induced cardiomyopathy: shorter protocol, reduced mortality, and higher incidence of overt dilated cardiomyopathy


Autoria(s): Talavera, Jesús; Giraldo, Alejandro; Fernández-Del-Palacio, María Josefa; García-Nicolás, Obdulio; Seva, Juan; Brooks, Gavin; Moraleda, Jose M.
Data(s)

26/11/2015

Resumo

Current protocols of anthracycline-induced cardiomyopathy in rabbits present with high premature mortality and nephrotoxicity, thus rendering them unsuitable for studies requiring long-term functional evaluation of myocardial function (e.g., stem cell therapy). We compared two previously described protocols to an in-house developed protocol in three groups: Group DOX2 received doxorubicin 2 mg/kg/week (8 weeks); Group DAU3 received daunorubicin 3 mg/kg/week (10 weeks); and Group DAU4 received daunorubicin 4 mg/kg/week (6 weeks). A cohort of rabbits received saline (control). Results of blood tests, cardiac troponin I, echocardiography, and histopathology were analysed. Whilst DOX2 and DAU3 rabbits showed high premature mortality (50% and 33%, resp.), DAU4 rabbits showed 7.6% premature mortality. None of DOX2 rabbits developed overt dilated cardiomyopathy; 66% of DAU3 rabbits developed overt dilated cardiomyopathy and quickly progressed to severe congestive heart failure. Interestingly, 92% of DAU4 rabbits showed overt dilated cardiomyopathy and 67% developed congestive heart failure exhibiting stable disease. DOX2 and DAU3 rabbits showed alterations of renal function, with DAU3 also exhibiting hepatic function compromise. Thus, a shortened protocol of anthracycline-induced cardiomyopathy as in DAU4 group results in high incidence of overt dilated cardiomyopathy, which insidiously progressed to congestive heart failure, associated to reduced systemic compromise and very low premature mortality.

Formato

text

Identificador

http://centaur.reading.ac.uk/51844/1/465342.pdf

Talavera, J., Giraldo, A. <http://centaur.reading.ac.uk/view/creators/90003687.html>, Fernández-Del-Palacio, M. J., García-Nicolás, O., Seva, J., Brooks, G. <http://centaur.reading.ac.uk/view/creators/90000316.html> and Moraleda, J. M. (2015) An upgrade on the rabbit model of anthracycline-induced cardiomyopathy: shorter protocol, reduced mortality, and higher incidence of overt dilated cardiomyopathy. BioMed research international, 2015. 465342. ISSN 2314-6133 doi: 10.1155/2015/465342 <http://dx.doi.org/10.1155/2015/465342>

Idioma(s)

en

Publicador

Hindawi

Relação

http://centaur.reading.ac.uk/51844/

creatorInternal Giraldo, Alejandro

creatorInternal Brooks, Gavin

http://dx.doi.org/10.1155/2015/465342

10.1155/2015/465342

Direitos

cc_by_4

Tipo

Article

PeerReviewed