A self-assembling fluorescent dipeptide conjugate for cell labelling


Autoria(s): Kirkham, Steven; Hamley, Ian W.; Smith, Andrew M.; Gouveia, Ricardo M.; Connon, Che J.; Reza, Mehedi; Ruokolainen, Janne
Data(s)

01/01/2016

Resumo

Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in peri-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers.

Formato

text

text

Identificador

http://centaur.reading.ac.uk/41591/8/1-s2.0-S0927776515002817-main.pdf

http://centaur.reading.ac.uk/41591/1/1-s2.0-S0927776515002817-main.pdf

Kirkham, S., Hamley, I. W. <http://centaur.reading.ac.uk/view/creators/90000472.html>, Smith, A. M. <http://centaur.reading.ac.uk/view/creators/90005142.html>, Gouveia, R. M. <http://centaur.reading.ac.uk/view/creators/90004572.html>, Connon, C. J. <http://centaur.reading.ac.uk/view/creators/90000658.html>, Reza, M. and Ruokolainen, J. (2016) A self-assembling fluorescent dipeptide conjugate for cell labelling. Colloids and Surfaces B: Biointerfaces, 137. pp. 104-108. ISSN 0927-7765 doi: 10.1016/j.colsurfb.2015.04.062 <http://dx.doi.org/10.1016/j.colsurfb.2015.04.062>

Idioma(s)

en

en

Publicador

Elsevier

Relação

http://centaur.reading.ac.uk/41591/

creatorInternal Hamley, Ian W.

creatorInternal Smith, Andrew M.

creatorInternal Gouveia, Ricardo M.

creatorInternal Connon, Che J.

http://dx.doi.org/10.1016/j.colsurfb.2015.04.062

10.1016/j.colsurfb.2015.04.062

Direitos

cc_by_4

cc_by_4

Tipo

Article

PeerReviewed