Characterization of virus-like particles associated with the human faecal and caecal microbiota
| Data(s) |
01/12/2014
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| Resumo |
This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid. |
| Formato |
text |
| Identificador |
http://centaur.reading.ac.uk/37814/1/Hoyles_accepted.pdf Hoyles, L., McCartney, A. L. <http://centaur.reading.ac.uk/view/creators/90000345.html>, Neve, H., Gibson, G. R. <http://centaur.reading.ac.uk/view/creators/90000347.html>, Sanderson, J. D., Heller, K. J. and van Sinderen, D. (2014) Characterization of virus-like particles associated with the human faecal and caecal microbiota. Research in Microbiology, 165 (10). pp. 803-812. ISSN 0923-2508 doi: 10.1016/j.resmic.2014.10.006 <http://dx.doi.org/10.1016/j.resmic.2014.10.006> |
| Idioma(s) |
en |
| Publicador |
Elsevier |
| Relação |
http://centaur.reading.ac.uk/37814/ creatorInternal McCartney, A. L. creatorInternal Gibson, G. R. 10.1016/j.resmic.2014.10.006 |
| Tipo |
Article PeerReviewed |
