Method for the production of cotton somatic embryos. United States Patent Application


Autoria(s): Dunwell, J.M.; Keith, D.J.; Nobre, J.M.P.
Data(s)

2003

Resumo

The present invention provides Inter alia, a method for the production of cotton somatic embryos comprising (a) isolating a totipotent stomatal cell-containing epidermal explant from leaf material excised from a cotton plant; and (b) culturing said explant in a basal medium which comprises an embryogenic callus-inducing quantity of an auxin and a cytokinin under an embryogenic callus inducing intensity of light until embryogenic callus is formed; and (c) sub-culturing said embryogenic callus onto a somatic embryo differentiation media to produce said somatic embryos. Plants may be regenerated from the somatic embryos and in a particular embodiment of the invention said totipotent stomatal cell is transformed, prior to the inducement of embryogenic callus, with a polynucleotide that provides for a desired agronomic trait.

Formato

text

Identificador

http://centaur.reading.ac.uk/10724/1/US20030143744.pdf

Syngenta Biotechnology Inc (2003) Method for the production of cotton somatic embryos. United States Patent Application. US20030143744. doi: US20030143744

Idioma(s)

en

Relação

http://centaur.reading.ac.uk/10724/

creatorInternal Dunwell, J.M.

US20030143744

Tipo

Patent

NonPeerReviewed