Method for the production of cotton somatic embryos. United States Patent Application
Data(s) |
2003
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Resumo |
The present invention provides Inter alia, a method for the production of cotton somatic embryos comprising (a) isolating a totipotent stomatal cell-containing epidermal explant from leaf material excised from a cotton plant; and (b) culturing said explant in a basal medium which comprises an embryogenic callus-inducing quantity of an auxin and a cytokinin under an embryogenic callus inducing intensity of light until embryogenic callus is formed; and (c) sub-culturing said embryogenic callus onto a somatic embryo differentiation media to produce said somatic embryos. Plants may be regenerated from the somatic embryos and in a particular embodiment of the invention said totipotent stomatal cell is transformed, prior to the inducement of embryogenic callus, with a polynucleotide that provides for a desired agronomic trait. |
Formato |
text |
Identificador |
http://centaur.reading.ac.uk/10724/1/US20030143744.pdf Syngenta Biotechnology Inc (2003) Method for the production of cotton somatic embryos. United States Patent Application. US20030143744. doi: US20030143744 |
Idioma(s) |
en |
Relação |
http://centaur.reading.ac.uk/10724/ creatorInternal Dunwell, J.M. US20030143744 |
Tipo |
Patent NonPeerReviewed |