Coloraciones histológicas vs microscopia electrónica de transmision para detección de Biopeliculas en pacientes con Rinosinusitis Crónica en el Hospital Universitario de la Samaritana en el año 2011
| Contribuinte(s) |
Gutierrez Guauque, Omar Alberto |
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| Data(s) |
03/09/2013
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| Resumo |
Objetivo: Determinar la concordancia entre microscopia de luz vs microscopia electrónica de transmisión para la detección de Biopeliculas en pacientes con Rinosinusitis Crónica Diseño: Estudio de concordancia. Materiales y Métodos: Analizamos 34 muestras de pacientes llevados a Cirugía Endoscópica Funcional por Rinosinusitis Crónica. Fueron procesadas para valoración mediante microscopia de luz usando Hematoxilina-Eosina, Gram, Acido Peryódico de Schiff, Giemsa y Microscopia Electrónica de Transmisión (MET). Resultados: No se identificaron Biopelícula en ninguna de las muestras analizadas bajo Microscopía Electrónica de Transmisión (MET), estos resultados son concordantes con los resultados obtenidos con las coloraciones histológicas Hematoxilina-Eosina (H-E), Gram, Giemsa y Acido Peryódico de Schiff (PAS), mostrando una concordancia absoluta con test de Kappa para resultados negativos del 100%. Conclusión: Existe una alta concordancia entre los hallazgos observados entre la MET y la Microscopia de luz para los resultados negativos Hospital Universitario de la Samaritana Objectives: Determine the concordance between light microscopy vs transmission electron microscopy for the detection of Biofilms in patients with Chronic Rhinosinusitis Materials and Methods: We analyzed 34 samples of patients who underwent Functional Endoscopic Surgery for Chronic Rhinosinusitis. These were processed for evaluation by light microscopy using Hematoxylin-Eosin, Gram, periodic acid-Schiff, Giemsa and Transmission Electron Microscopy (TEM). Results: Biofilm was not identified in any of the samples analyzed under transmission electron microscopy (TEM), these results are consistent with the results obtained with hematoxylin-eosin histological staining (HE), Gram, Giemsa and periodic acid-Schiff (PAS), showing an absolute concordance with Kappa test of 100% for negative results. Conclusion: There is a high correlation between the findings observed between the TEM and light microscopy for the negative results Clinical significance: Create a new tool for the diagnosis of Biofilms. |
| Formato |
application/pdf |
| Identificador | |
| Idioma(s) |
spa |
| Publicador |
Facultad de medicina |
| Direitos |
info:eu-repo/semantics/openAccess |
| Fonte |
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| Palavras-Chave | #Otorrinolaringología #610 #Sinusitis Crónica #Microscopia Electrónica #Histología - Técnicas #Biofilms #Chronic Sinusitis #Histological techniques #Electron Microscopy |
| Tipo |
info:eu-repo/semantics/bachelorThesis |
