Impact of process parameters on L-glutaminase production by marine Vibrio costicola in solid state fermentation using polystyrene as an inert support


Autoria(s): Chandrasekaran, M; Nagendra, Prabhu G
Data(s)

23/07/2014

23/07/2014

1997

Resumo

Process parameters influencing e-glutaminase production by marine Vibrio costicola in solid state fermentation (SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g dry substrate) was obtained at 2% (w/w) t:glutamine, 35°C and pH 7.0 after 24 h. Maltose and potassium dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4.2 U/mg protein) and low viscosity (0-966 Ns/m 2) was recovered from the polystyrene SSF system

Process Biochemistry, Vol. 32, No. 4, pp. 285-289, 1997

Cochin University of Science and Technology

Identificador

http://dyuthi.cusat.ac.in/purl/4243

Idioma(s)

en

Publicador

Elsevier

Palavras-Chave #L-glutaminase #inert support #marine Vibrio costicola #polystyrene #solid state fermentation #process parameters.
Tipo

Article