Impact of process parameters on L-glutaminase production by marine Vibrio costicola in solid state fermentation using polystyrene as an inert support
Data(s) |
23/07/2014
23/07/2014
1997
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Resumo |
Process parameters influencing e-glutaminase production by marine Vibrio costicola in solid state fermentation (SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g dry substrate) was obtained at 2% (w/w) t:glutamine, 35°C and pH 7.0 after 24 h. Maltose and potassium dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4.2 U/mg protein) and low viscosity (0-966 Ns/m 2) was recovered from the polystyrene SSF system Process Biochemistry, Vol. 32, No. 4, pp. 285-289, 1997 Cochin University of Science and Technology |
Identificador | |
Idioma(s) |
en |
Publicador |
Elsevier |
Palavras-Chave | #L-glutaminase #inert support #marine Vibrio costicola #polystyrene #solid state fermentation #process parameters. |
Tipo |
Article |