Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus


Autoria(s): Gandra,Eliezer Avila; Fernandez,Maria Aparecida; Silva,Jorge Adolfo; Silva,Wladimir Padilha da
Data(s)

01/12/2011

Resumo

The enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1.

Formato

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Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019

Idioma(s)

en

Publicador

Sociedade Brasileira de Ciência e Tecnologia de Alimentos

Fonte

Food Science and Technology (Campinas) v.31 n.4 2011

Palavras-Chave #S. aureus #S. hyicus #S. intermedius #PCR #nuc gene
Tipo

journal article