Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation


Autoria(s): Osuna,M.D.; Goulart,I.C.G.R.; Vidal,R.A.; Kalsing,A.; Ruiz Santaella,J.P.; De Prado,R.
Data(s)

01/09/2012

Resumo

Eleusine indica (goosegrass) is a diploid grass weed which has developed resistance to ACCase inhibitors during the last ten years due to the intensive and frequent use of sethoxydim to control grass weeds in soybean crops in Brazil. Plant dose-response assays confirmed the resistant behaviour of one biotype obtaining high resistance factor values: 143 (fenoxaprop), 126 (haloxyfop), 84 (sethoxydim) to 58 (fluazifop). ACCase in vitro assays indicated a target site resistance as the main cause of reduced susceptibility to ACCase inhibitors. PCR-generated fragments of the ACCase CT domain of the resistant and sensitive reference biotype were sequenced and compared. A point mutation was detected within the triplet of aspartate at the amino acid position 2078 (referred to EMBL accession no. AJ310767) and resulted in the triplet of glycine. These results constitute the first report on a target site mutation for a Brazilian herbicide resistant grass weed.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000300025

Idioma(s)

en

Publicador

Sociedade Brasileira da Ciência das Plantas Daninhas

Fonte

Planta Daninha v.30 n.3 2012

Palavras-Chave #goosegrass #cross-resistance #enzyme #PCR #herbicide
Tipo

journal article