Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered in abattoirs in Pernambuco, Brazil


Autoria(s): Bezerra,Mauro J.G.; Cruz,Jefferson A.L.O.; Kung,Eugênio S.; Silva,José G.; Santos,André S.; Moraes,Érica P.B.X.; Pinheiro Junior,José W.; Mota,Rinaldo A.
Data(s)

01/04/2014

Resumo

The aim of the present study was to assess the occurrence of antibodies to Toxoplasma gondii and to detect genomic DNA of the parasite in the reproductive organs, fetuses and fetal membranes of sheep in slaughterhouses in the state of Pernambuco, Brazil. The Indirect Immunofluorescence technique (IFA) was used for screening. The Polymerase Chain Reaction (PCR) was used to detect DNA of T. gondii in the animals that were positive in the serology. In the serology, 13/50 samples were positive and genomic DNA of T. gondii was detected in one uterus, tube, ovary, placenta and fetus (heart, brain and umbilical cord) sample from a sheep that was positive in the serology. The present study provides evidence of the occurrence of T. gondii DNA in the organs of the reproductive system, placenta and fetus of a naturally infected sheep.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2014000400005

Idioma(s)

en

Publicador

Colégio Brasileiro de Patologia Animal - CBPA. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)

Fonte

Pesquisa Veterinária Brasileira v.34 n.4 2014

Palavras-Chave #Toxoplasmosis #Toxoplasma gondii #sheep #Polymerase Chain Reaction
Tipo

journal article