Effect of bone morphogenetic protein-7 (BMP-7) on in vitro survival of caprine preantral follicles


Autoria(s): Araújo,Valdevane R.; Silva,Cleidson M. Gomes da; Magalhães,Deborah M.; Silva,Gerlane Modesto da; Báo,Sônia N.; Silva,José Roberto Viana; Figueiredo,José Ricardo de; Rodrigues,Ana Paula R.
Data(s)

01/04/2010

Resumo

This study was conducted in order to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) supplemented with different concentrations of BMP-7 (1, 10, 50 or 100ng/ml). Non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM). Parameters such as follicular survival, activation and growth were evaluated. The results showed that, after 1 or 7 days of culture, the percentage of morphologically normal follicles was significantly reduced in all treatments when compared with fresh control, except at 1ng/ml of BMP-7 for 1 day. In addition, the concentration of 10ng/ml of BMP-7 significantly increases follicular diameter from day 1 to 7 of culture. There was no influence of the other concentrations of BMP-7 regarding to the follicular and oocyte diameter. Ultrastructure studies confirmed follicular integrity after 7 days of culture in 1ng/ml BMP-7. In conclusion, small concentrations of BMP-7 can improve the survival and growth of caprine preantral follicles during in vitro culture.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010000400004

Idioma(s)

en

Publicador

Colégio Brasileiro de Patologia Animal - CBPA. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)

Fonte

Pesquisa Veterinária Brasileira v.30 n.4 2010

Palavras-Chave #Ovary #preantral follicles #growth factor #bone morphogenetic protein-7 #BMP-7 #goats
Tipo

journal article