Identification of the recently described new type of bovine papillomavirus (BPV-8) in a Brazilian beef cattle herd


Autoria(s): Claus,Marlise P.; Lunardi,Michele; Alfieri,Alice F.; Sartori,Daniele; Fungaro,Maria Helena P.; Alfieri,Amauri A.
Data(s)

01/01/2009

Resumo

Bovine papillomavirus type 8 (BPV-8) was first detected and described in teat warts as well as in healthy teat skin from cattle raised in Japan. The entire viral genome was sequenced in 2007. Additionally, a variant of BPV-8, BPV-8-EB, was also identified from papillomatous lesions of a European bison in Slovakia. In Brazil, despite the relatively common occurrence of BPV infections, the identification and determination of viral types present in cattle is still sporadic. The aim of this study is to report the occurrence of the recently described BPV-8 in Brazil. The virus was identified in a skin warts obtained from a beef cattle herd located in Parana state, southern Brazil. The papilloma had a macular, non-verrucous gross aspect and was located on the dorsal thorax of a cow. Polymerase chain reaction (PCR) was performed using generic primers for partial amplification of L1 gene. The obtained amplicon (480bp) was cloned and two selected clones were sequenced. The nucleotide sequence was compared to existing papillomaviral genomic sequences, identifying the virus as BPV type 8. This study represents the first report of BPV-8 occurrence in Brazil, what suggests its presence among Brazilian cattle.

Formato

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Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2009000100003

Idioma(s)

en

Publicador

Colégio Brasileiro de Patologia Animal - CBPA. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)

Fonte

Pesquisa Veterinária Brasileira v.29 n.1 2009

Palavras-Chave #Bovine #bovine papillomavirus type 8 #BPV-8 #cutaneous papillomatosis #molecular analysis
Tipo

journal article