Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs


Autoria(s): Minho,Alessandro Pelegrine; Freire,Roberta Lemos; Vidotto,Odilon; Gennari,Solange Maria; Marana,Elizabete Marangoni; Garcia,João Luis; Navarro,Italmar Teodorico
Data(s)

01/12/2004

Resumo

The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2004000400005

Idioma(s)

en

Publicador

Colégio Brasileiro de Patologia Animal - CBPA. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)

Fonte

Pesquisa Veterinária Brasileira v.24 n.4 2004

Palavras-Chave #Toxoplasma gondii #swine #antibodies #serology #indirect fluorescent antibody test (IFAT) #modified agglutination test (MAT)
Tipo

journal article