Composição química e atividade anticolinesterásica de uma fração ativa do extrato de folhas de Citrus limon (L.) Burm


Autoria(s): Carvalho,Rusbene Bruno Fonseca de; Almeida,Antonia Amanda Cardoso de; Freitas,Rivelilson Mendes de; Lima,Luciano Silva; David,Juceni Pereira; David,Jorge Mauricio; Feitosa,Chistiane Mendes
Data(s)

01/01/2013

Resumo

This paper describes the chemical composition and acetylcholinesterase inhibition of an active chromatographic fraction of the EtOAc extract of Citrus limon leaves. The composition of the active fraction presented a mixture of two coumarins, 5,8-dimethoxy-psoralen and 5,7- dimethoxycoumarin, identified by ¹H and ¹³C NMR data analysis, including DEPT, COSY, HMBC and HSQC experiments. It was also demonstrated that this mixture presents qualitative and quantitative AChE inhibition. In vitro studies indicated a CE50 value of 340 µg/mL with 95% of confidence. In vivo studies (10 and 25 mg/kg) revealed inhibition of 30.09 and 30.06% of AChE activity in relation to neostigmine, respectively.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000900017

Idioma(s)

pt

Publicador

Sociedade Brasileira de Química

Fonte

Química Nova v.36 n.9 2013

Palavras-Chave #Citrus limon (L.) Burm #psoraleno #acetylcholinesterase
Tipo

journal article