Validação de método e determinação espectrométrica dos flavonoides das folhas e do vinhoto da cana-de-açúcar e comparação com método CLAE-UV

This paper reports on a modification of the procedures originally described in the French Pharmacopoeia for the UV-visible spectrometric analysis of flavonoids, and proposes a validation of the method and its application in the determination of total flavonoids from sugarcane (Saccharum officinarum) leaves and vinasse. An analysis of precision and accuracy revealed a low relative standard deviation (< 5.0%) and a good recovery percentages (99.79 and 98.34%). A comparison of the spectrometric results against those obtained by high performance liquid chromatography (HPLC-UV) demonstrated complete compatibility between the modified French Pharmacopoeia (spectrometric) and HPLC-UV methods