Filmes ultrafinos de ésteres de celulose: preparo, caracterização e imobilização de proteínas


Autoria(s): Amim Jr.,Jorge; Petri,Denise F. S.; Maia,Francisco C. B.; Miranda,Paulo B.
Data(s)

01/01/2010

Resumo

In this study cellulose acetate butyrate (CAB) and carboxymehtylcellulose acetate butyrate (CMCAB) films adsorbed onto silicon wafers were characterized by means of ellipsometry, atomic force microscopy (AFM), sum frequency generation spectroscopy (SFG) and contact angle measurements. The adsorption behavior of lysozyme (LIS) or bovine serum albumin (BSA) onto CAB and CMCAB films was investigated. The amounts of adsorbed LIS or BSA onto CMCAB films were more pronounced than those onto CAB films due to the presence of carboxymethyl group in the CMCAB structure. Besides, the adsorption of BSA molecules on CMCAB films was more favored than that of LIS molecules. Antimicrobial effect of LIS bound to CAB or CMCAB layers was evaluated using Micrococcus luteus as substrate.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010001000011

Idioma(s)

pt

Publicador

Sociedade Brasileira de Química

Fonte

Química Nova v.33 n.10 2010

Palavras-Chave #cellulose ester #protein adsorption #film characterization
Tipo

journal article