Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten
Data(s) |
01/01/2010
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Resumo |
The Michaelis-Menten equation is used in many biochemical and bioinorganic kinetic studies involving homogeneous catalysis. Otherwise, it is known that determination of Michaelis-Menten parameters K M, Vmax, and k cat by the well-known Lineweaver-Burk double reciprocal linear equation does not produce the best values for these parameters. In this paper we present a discussion on different linear equations which can be used to calculate these parameters and we compare their results with the values obtained by the more reliable nonlinear least-square fit. |
Formato |
text/html |
Identificador |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700033 |
Idioma(s) |
pt |
Publicador |
Sociedade Brasileira de Química |
Fonte |
Química Nova v.33 n.7 2010 |
Palavras-Chave | #Michaelis-Menten #Lineweaver-Burk #nonlinear least-square fit |
Tipo |
journal article |