Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten


Autoria(s): Carvalho,Nakédia M. F.; Pires,Bianca M.; Antunes,Octavio A. C.; Faria,Roberto B.; Osório,Renata E. H. M. B.; Piovezan,Clovis; Neves,Ademir
Data(s)

01/01/2010

Resumo

The Michaelis-Menten equation is used in many biochemical and bioinorganic kinetic studies involving homogeneous catalysis. Otherwise, it is known that determination of Michaelis-Menten parameters K M, Vmax, and k cat by the well-known Lineweaver-Burk double reciprocal linear equation does not produce the best values for these parameters. In this paper we present a discussion on different linear equations which can be used to calculate these parameters and we compare their results with the values obtained by the more reliable nonlinear least-square fit.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700033

Idioma(s)

pt

Publicador

Sociedade Brasileira de Química

Fonte

Química Nova v.33 n.7 2010

Palavras-Chave #Michaelis-Menten #Lineweaver-Burk #nonlinear least-square fit
Tipo

journal article