UV derivative spectrophotometric method for determination of estradiol valerate in tablets


Autoria(s): Mendez,Andreas S. L.; Deconto,Lislaine; Garcia,Cássia V.
Data(s)

01/01/2010

Resumo

A derivative UV spectrophotometric method for determination of estradiol valerate in tablets was validated. The parameters specificity, linearity, precision, accuracy, limit of detection and limit of quantitation were studied according to validation guidelines. The first-order derivative spectra were obtained at N = 5, Δλ = 4.0 nm, and determinations were made at 270 nm. The method showed specificity and linearity in the concentration range of 0.20 to 0.40 mg mL-1. The intra and interday precision data demonstrated the method has good reproducibility. Accuracy was also evaluated and results were satisfactory. The proposed method was successfully applied to a pharmaceutical formulation.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000400040

Idioma(s)

en

Publicador

Sociedade Brasileira de Química

Fonte

Química Nova v.33 n.4 2010

Palavras-Chave #estradiol valerate #UV derivative spectrophotometric method #method validation
Tipo

journal article