Oligonucleotide cyclization: The thiol-maleimide reaction revisited
| Contribuinte(s) |
Universitat de Barcelona |
|---|---|
| Resumo |
A novel method to synthesize cyclic oligonucleotides (5- to 26-mer) using the thiol-maleimide reaction is described. The target molecules were obtained after subsequent removal of thiol and maleimide protecting groups from 5′-maleimido-3′-thiol-derivatized linear precursors. Retro-Diels-Alder conditions deprotecting the maleimide simultaneously promoted cyclization cleanly and in high yield. |
| Identificador | |
| Idioma(s) |
eng |
| Publicador |
Royal Society of Chemistry |
| Direitos |
(c) Sánchez, Albert et al., 2013 info:eu-repo/semantics/openAccess |
| Palavras-Chave | #Bioquímica #Àcids nucleics #Bacteris #Microbiologia #Ciclització (Química) #Biochemistry #Nucleic acids #Bacteria #Microbiology #Ring formation (Chemistry) |
| Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
