OmpA family proteins and Pmp-like autotransporter: new adhesins of Waddlia chondrophila.


Autoria(s): Kebbi-Beghdadi C.; Domröse A.; Becker E.; Cisse O.H.; Hegemann J.H.; Greub G.
Data(s)

2015

Resumo

Waddlia chondrophila is a obligate intracellular bacterium belonging to the Chlamydiales order, a clade that also includes the well-known classical Chlamydia responsible for a number of severe human and animal diseases. Waddlia is an emerging pathogen associated with adverse pregnancy outcomes in humans and abortion in ruminants. Adhesion to the host cell is an essential prerequisite for survival of every strict intracellular bacteria and, in classical Chlamydia, this step is partially mediated by polymorphic outer membrane proteins (Pmps), a family of highly diverse autotransporters that represent about 15% of the bacterial coding capacity. Waddlia chondrophila genome however only encodes one putative Pmp-like protein. Using a proteomic approach, we identified several bacterial proteins potentially implicated in the adhesion process and we characterized their expression during the replication cycle of the bacteria. In addition, we demonstrated that the Waddlia Pmp-like autotransporter as well as OmpA2 and OmpA3, two members of the extended Waddlia OmpA protein family, exhibit adhesive properties on epithelial cells. We hypothesize that the large diversity of the OmpA protein family is linked to the wide host range of these bacteria that are able to enter and multiply in various host cells ranging from protozoa to mammalian and fish cells.

Identificador

http://serval.unil.ch/?id=serval:BIB_CBA1FA9E394E

isbn:2049-632X (Electronic)

pmid:25986220

doi:10.1093/femspd/ftv035

isiid:000362574300004

Idioma(s)

en

Fonte

Pathogens and Disease, vol. 73, no. 6, pp. ftv035

Palavras-Chave #intracellular bacteria; adhesins; autotransporter; chlamydia; virulence
Tipo

info:eu-repo/semantics/article

article