Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.
Data(s) |
2009
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Resumo |
Rho GTPases regulate the actin cytoskeleton in all eukaryotes. Fission yeast Cdc42 is involved in actin cable assembly and formin For3 regulation. We isolated cdc42-879 as a thermosensitive strain with actin cable and For3 localization defects. In a multicopy suppressor screening, we identified pob1(+) as suppressor of cdc42-879 thermosensitivity. Pob1 overexpression also partially restores actin cables and localization of For3 in the mutant strain. Pob1 interacts with Cdc42 and this GTPase regulates Pob1 localization and/or stability. The C-terminal pleckstrin homology (PH) domain of Pob1 is required for Cdc42 binding. Pob1 also binds to For3 through its N-terminal sterile alpha motif (SAM) domain and contributes to the formin localization at the cell tips. The previously described pob1-664 mutant strain (Mol. Biol. Cell. 10, 2745-2757, 1999), which carries a mutation in the PH domain, as well as pob1 mutant strains in which Pob1 lacks the N-terminal region (pob1DeltaN) or the SAM domain (pob1DeltaSAM), have cytoskeletal defects similar to that of cdc42-879 cells. Expression of constitutively active For3DAD* partially restores actin organization in cdc42-879, pob1-664, pob1DeltaN, and pob1DeltaSAM. Therefore, we propose that Pob1 is required for For3 localization to the tips and facilitates Cdc42-mediated relief of For3 autoinhibition to stimulate actin cable formation. |
Identificador |
http://serval.unil.ch/?id=serval:BIB_F9055D3C4CB4 isbn:1939-4586 (Electronic) pmid:19710424 doi:10.1091/mbc.E09-03-0207 isiid:000270838700012 |
Idioma(s) |
en |
Fonte |
Molecular Biology of the Cell, vol. 20, no. 20, pp. 4390-4399 |
Palavras-Chave | #Actin Cytoskeleton/metabolism; Actin Cytoskeleton/ultrastructure; Actins/metabolism; Amino Acid Substitution; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/physiology; Cell Polarity; Cytoskeleton/ultrastructure; Gene Expression Regulation, Fungal; Guanosine Triphosphate/physiology; Protein Interaction Mapping; Protein Structure, Tertiary; Schizosaccharomyces/metabolism; Schizosaccharomyces/ultrastructure; Schizosaccharomyces pombe Proteins/chemistry; Schizosaccharomyces pombe Proteins/genetics; cdc42 GTP-Binding Protein/chemistry; cdc42 GTP-Binding Protein/genetics; rho GTP-Binding Proteins/chemistry; rho GTP-Binding Proteins/physiology |
Tipo |
info:eu-repo/semantics/article article |