New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples
| Data(s) |
2008
|
|---|---|
| Resumo |
Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis. |
| Identificador |
http://serval.unil.ch/?id=serval:BIB_F8EF3D970451 isbn:1098-660X pmid:18234873 doi:10.1128/JCM.02302-07 isiid:000254866400050 |
| Idioma(s) |
en |
| Fonte |
Journal of Clinical Microbiology, vol. 46, no. 4, pp. 1491-1493 |
| Palavras-Chave | #Acanthamoeba ; Adolescent ; analysis ; Animals ; Bacteria ; Bronchiolitis ; Child,Preschool ; Chlamydiales ; diagnosis ; Dna ; DNA,Bacterial ; Female ; genetics ; Gram-Negative Bacterial Infections ; Humans ; Infant ; isolation & purification ; Male ; methods ; microbiology ; Patients ; Polymerase Chain Reaction ; Reproducibility of Results ; Role ; Sensitivity and Specificity ; Switzerland |
| Tipo |
info:eu-repo/semantics/article article |
