Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines.


Autoria(s): Accolla R.S.; Carrel S.; Mach J.P.
Data(s)

1980

Resumo

Spleen cells from mice immunized with purified carcinoembryonic antigen (CEA), an important tumor marker of human carcinomas, were fused with the mouse myeloma cell line P3-NSI/1-Ag4. Out of the 400 hybrids obtained, 2 secreted antibodies reacting specifically with two different antigenic determinants present on CEA molecules. They were cloned and established as permanent hybridoma cell lines. These antibodies, which have relatively high-affinities and can be produced in unlimited amounts, will be useful both for the immunochemical characterization of CEA and as a standard reagent for the identification of this antigen in human tissues and body fluids.

Identificador

http://serval.unil.ch/?id=serval:BIB_F10530E4A862

isbn:0027-8424 (Print)

pmid:6153797

doi:10.1073/pnas.77.1.563

isiid:A1980JL81200107

Idioma(s)

en

Fonte

Proceedings of the National Academy of Sciences of the United States of America, vol. 77, no. 1, pp. 563-566

Palavras-Chave #Animals; Antibodies, Neoplasm/isolation & purification; Antibody Specificity; Binding Sites, Antibody; Carcinoembryonic Antigen; Clone Cells/immunology; Epitopes; Hybrid Cells/immunology; Immunoglobulin Allotypes; Immunoglobulin G; Kinetics; Mice
Tipo

info:eu-repo/semantics/article

article