Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines.
Data(s) |
1980
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Resumo |
Spleen cells from mice immunized with purified carcinoembryonic antigen (CEA), an important tumor marker of human carcinomas, were fused with the mouse myeloma cell line P3-NSI/1-Ag4. Out of the 400 hybrids obtained, 2 secreted antibodies reacting specifically with two different antigenic determinants present on CEA molecules. They were cloned and established as permanent hybridoma cell lines. These antibodies, which have relatively high-affinities and can be produced in unlimited amounts, will be useful both for the immunochemical characterization of CEA and as a standard reagent for the identification of this antigen in human tissues and body fluids. |
Identificador |
http://serval.unil.ch/?id=serval:BIB_F10530E4A862 isbn:0027-8424 (Print) pmid:6153797 doi:10.1073/pnas.77.1.563 isiid:A1980JL81200107 |
Idioma(s) |
en |
Fonte |
Proceedings of the National Academy of Sciences of the United States of America, vol. 77, no. 1, pp. 563-566 |
Palavras-Chave | #Animals; Antibodies, Neoplasm/isolation & purification; Antibody Specificity; Binding Sites, Antibody; Carcinoembryonic Antigen; Clone Cells/immunology; Epitopes; Hybrid Cells/immunology; Immunoglobulin Allotypes; Immunoglobulin G; Kinetics; Mice |
Tipo |
info:eu-repo/semantics/article article |