Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro.
| Data(s) |
1999
|
|---|---|
| Resumo |
Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage lambda in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21. |
| Identificador |
http://serval.unil.ch/?id=serval:BIB_EEDAED4BA635 isbn:0021-9193 (Print) pmid:10094711 isiid:000079368400040 |
| Idioma(s) |
en |
| Fonte |
Journal of Bacteriology, vol. 181, no. 7, pp. 2286-2289 |
| Palavras-Chave | #Artificial Gene Fusion; Bacterial Proteins/physiology; Bacteriophage lambda/genetics; DNA Transposable Elements; Escherichia coli/genetics; Replicon; Tandem Repeat Sequences |
| Tipo |
info:eu-repo/semantics/article article |
