The fusion protein of wild-type canine distemper virus is a major determinant of persistent infection.


Autoria(s): Plattet P.; Rivals J.P.; Zuber B.; Brunner J.M.; Zurbriggen A.; Wittek R.
Data(s)

01/07/2005

Resumo

The wild-type A75/17 canine distemper virus (CDV) strain induces a persistent infection in the central nervous system but infects cell lines very inefficiently. In contrast, the genetically more distant Onderstepoort CDV vaccine strain (OP-CDV) induces extensive syncytia formation. Here, we investigated the roles of wild-type fusion (F(WT)) and attachment (H(WT)) proteins in Vero cells expressing, or not, the canine SLAM receptor by transfection experiments and by studying recombinants viruses expressing different combinations of wild-type and OP-CDV glycoproteins. We show that low fusogenicity is not due to a defect of the envelope proteins to reach the cell surface and that H(WT) determines persistent infection in a receptor-dependent manner, emphasizing the role of SLAM as a potent enhancer of fusogenicity. However, importantly, F(WT) reduced cell-to-cell fusion independently of the cell surface receptor, thus demonstrating that the fusion protein of the neurovirulent A75/17-CDV strain plays a key role in determining persistent infection.

Identificador

http://serval.unil.ch/?id=serval:BIB_E750637DDD09

isbn:0042-6822[print], 0042-6822[linking]

pmid:15893783

doi:10.1016/j.virol.2005.04.012

isiid:000230155300012

Idioma(s)

en

Fonte

Virology, vol. 337, no. 2, pp. 312-326

Palavras-Chave #Amino Acids/analysis; Animals; Biotinylation; Cercopithecus aethiops; Distemper/virology; Distemper Virus, Canine/pathogenicity; Dogs; Fluorescent Antibody Technique, Indirect; Transfection; Vero Cells; Viral Fusion Proteins/analysis; Viral Fusion Proteins/chemistry
Tipo

info:eu-repo/semantics/article

article