Preferential infection of immature dendritic cells and B cells by mouse mammary tumor virus.
Data(s) |
2002
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Resumo |
Until now it was thought that the retrovirus mouse mammary tumor virus preferentially infects B cells, which thereafter proliferate and differentiate due to superantigen-mediated T cell help. We describe in this study that dendritic cells are infectable at levels comparable to B cells in the first days after virus injection. Moreover, IgM knockout mice have chronically deleted superantigen-reactive T cells after MMTV injection, indicating that superantigen presentation by dendritic cells is sufficient for T cell deletion. In both subsets initially only few cells were infected, but there was an exponential increase in numbers of infected B cells due to superantigen-mediated T cell help, explaining that at the peak of the response infection is almost exclusively found in B cells. The level of infection in vivo was below 1 in 1000 dendritic cells or B cells. Infection levels in freshly isolated dendritic cells from spleen, Langerhans cells from skin, or bone marrow-derived dendritic cells were compared in an in vitro infection assay. Immature dendritic cells such as Langerhans cells or bone marrow-derived dendritic cells were infected 10- to 30-fold more efficiently than mature splenic dendritic cells. Bone marrow-derived dendritic cells carrying an endogenous mouse mammary tumor virus superantigen were highly efficient at inducing a superantigen response in vivo. These results highlight the importance of professional APC and efficient T cell priming for the establishment of a persistent infection by mouse mammary tumor virus. |
Identificador |
http://serval.unil.ch/?id=serval:BIB_E08993B57D6F isbn:0022-1767[print], 0022-1767[linking] pmid:11907107 isiid:000174566400047 |
Idioma(s) |
en |
Fonte |
Journal of Immunology, vol. 168, no. 7, pp. 3470-3476 |
Palavras-Chave | #Adoptive Transfer; Animals; Antigen Presentation/genetics; B-Lymphocyte Subsets/immunology; B-Lymphocyte Subsets/pathology; CD4-Positive T-Lymphocytes/immunology; CD4-Positive T-Lymphocytes/metabolism; Cell Differentiation/genetics; Cell Differentiation/immunology; Cells, Cultured; Clonal Deletion/genetics; Dendritic Cells/immunology; Dendritic Cells/pathology; Interphase/immunology; Lymph Nodes/immunology; Lymph Nodes/pathology; Lymphopenia/genetics; Lymphopenia/immunology; Mammary Tumor Virus, Mouse/immunology; Mice; Mice, Inbred BALB C; Mice, Inbred DBA; Mice, Knockout; Retroviridae Infections/immunology; Superantigens/biosynthesis; Superantigens/immunology; Tumor Virus Infections/immunology |
Tipo |
info:eu-repo/semantics/article article |